UNASSIGNED: HepG2 cells were treated with Gal in the presence or absence of EX-527, a SIRT1-specific inhibitor, for 24 h. Sirtuin1 gene and protein expression were measured by RT-PCR and Western blotting, respectively. It has been docked to the allosteric reign of SIRT1 (PDB ID: 4ZZJ) to study the effect of Gal on SIRT1, and then the protein and complex molecular dynamic (MD) simulations had been studied in 100 ns.
UNASSIGNED: The semi-quantitative results of Oil red (p < .03) and TG level (p < .009) showed a significant reduction in lipid accumulation by treatment with Gal. Also, a significant increase was observed in the gene and protein expression of SIRT1 (p < .05). MD studies have shown that the average root mean square deviation (RMSD) was about 0.51 Å for protein structure and 0.66 Å for the complex. The average of radius of gyration (Rg) is 2.33 and 2.32 Å for protein and complex, respectively, and the pattern of root mean square fluctuation (RMSF) was almost similar.
UNASSIGNED: Computational studies show that Gal can be a great candidate to use as a SIRT1 ligand because it does not interfere with the structure of the protein, and other experimental studies showed that Gal treatment with SIRT1 inhibitor increases fat accumulation in HepG2 cells.
■在存在或不存在SIRT1特异性抑制剂EX-527的情况下,用Gal处理HepG2细胞,24小时。通过RT-PCR和Western印迹测量Sirtuin1基因和蛋白质表达,分别。已将其与SIRT1的变构统治(PDBID:4ZZJ)对接,以研究Gal对SIRT1的影响,然后在100ns内研究了蛋白质和复杂分子动力学(MD)模拟。
■油红(p<.03)和TG水平(p<.009)的半定量结果显示,通过用Gal处理,脂质积累显着减少。此外,SIRT1的基因和蛋白表达显著增加(p<0.05)。MD研究表明,蛋白质结构的平均均方根偏差(RMSD)约为0.51和复合物的0.66。蛋白质和复合物的平均回转半径(Rg)为2.33和2.32µ,分别,均方根波动(RMSF)的模式几乎相似。
■计算研究表明,Gal可以作为SIRT1配体使用,因为它不会干扰蛋白质的结构,和其他实验研究表明,用SIRT1抑制剂Gal处理增加脂肪在HepG2细胞中的积累。