PDF(Pubmed)
Abstract：
Diarrhea is a common gastrointestinal disorder in horses, with diet-induced diarrhea being an emerging challenge. This study aimed to investigate the gut microbiota differences in healthy and diet-induced diarrheic horses and evaluate the effectiveness of fecal microbiota transplantation (FMT) and carbonate buffer mixture (CBM) as potential therapeutic approaches. Twenty healthy horses were included in the study, with four groups: Control, Diarrhea, CBM, and FMT. Diarrhea was induced using oligofructose, and fecal samples were collected for microbiota analysis. FMT and CBM treatments were administered orally using donor fecal matter, and formula mixture, respectively. Clinical parameters, serum levels, intestinal tissue histopathology, and fecal microbiota profiles were evaluated. The results showed that diarrhea induction disbalanced the gut microbiota with decreased diversity and richness, affected clinical parameters including elevated body temperature and diarrhea score, and decreased fecal pH, increased inflammatory responses such as increased serum LPS, IL-17A, lactic acid and total protein, and caused damage in the colon tissue. CBM and FMT treatments altered the gut microbiota composition, restoring it towards a healthier profile compared to diarrheic, restored the gut microbiota composition to healthier states, improved clinical symptoms including decreased body temperature and diarrhea score, and increased fecal pH, decreased inflammatory responses such as increased serum LPS, IL-17A, lactic acid and total protein, and repaired tissue damage. CBM and FMT Spearman correlation analysis identified specific bacterial taxa associated with host parameters and inflammation. FMT and CBM treatments showed promising therapeutic effects in managing oligofructose-induced diarrhea in horses. The findings provide valuable insights into the management and treatment of diarrhea in horses and suggest the potential of combined CBM and FMT approaches for optimal therapeutic outcomes.
摘要：
腹泻是马常见的胃肠道疾病，饮食引起的腹泻是一个新兴的挑战。本研究旨在调查健康和饮食诱导的腹泻马的肠道微生物群差异，并评估粪便微生物群移植（FMT）和碳酸盐缓冲液混合物（CBM）作为潜在治疗方法的有效性。20匹健康的马被纳入研究，有四组：控制，腹泻,CBM,和FMT。腹泻是用低聚果糖诱导的，收集粪便样品进行微生物群分析。使用供体粪便物质口服FMT和CBM治疗，和配方混合物，分别。临床参数,血清水平，肠组织病理学，和粪便微生物区系进行了评估。结果表明，腹泻诱导使肠道菌群失衡，多样性和丰富度降低，受影响的临床参数，包括体温升高和腹泻评分，粪便pH值下降，炎症反应增加，如血清LPS增加，IL-17A,乳酸和总蛋白质，造成结肠组织损伤.CBM和FMT治疗改变了肠道菌群组成，与腹泻相比，将其恢复到更健康的状态，将肠道微生物群组成恢复到更健康的状态，改善临床症状，包括体温下降和腹泻评分，增加粪便的pH值,炎症反应减少，如血清LPS增加，IL-17A,乳酸和总蛋白质，修复组织损伤。CBM和FMTSpearman相关性分析确定了与宿主参数和炎症相关的特定细菌类群。FMT和CBM治疗在管理马的低聚果糖引起的腹泻方面显示出有希望的治疗效果。这些发现为马腹泻的管理和治疗提供了有价值的见解，并提出了CBM和FMT联合方法对最佳治疗结果的潜力。
