PDF(Pubmed)
Abstract：
UNASSIGNED: Borrelia miyamotoi is a spirochete species transmitted via hard ticks. Following its discovery in Japan, this pathogen has been detected around the world, and is increasingly confirmed as a human pathogen causing febrile disease, namely relapsing fever. Its presence has been confirmed in the Northeast China. However, there is little information regarding the presence of B. miyamotoi and other hard-tick-borne relapsing fever spirochetes in southern China including Yunnan province, where tick and animal species are abundant and many people both inhabit and visit for recreation.
UNASSIGNED: For the present study, we collected samples of ticks, wildlife, and domestic animal hosts from different counties in Yunnan province. Nucleic acids from samples were extracted, and the presence of B. miyamotoi and other relapsing fever spirochetes was confirmed using polymerase chain reaction (PCR) for the 16S rRNA specific target gene fragment. The positive samples were then amplified for partial genome of the flaB and glpQ genes. Statistical differences in its distribution were analyzed by SPSS 20 software. Sequence of partial 16S rRNA, flaB and glpQ genome were analyzed and phylogenetic trees were constructed.
UNASSIGNED: A total of 8260 samples including 2304 ticks, 4120 small mammals and 1836 blood of domestic animal hosts were collected for screening for infection of B. miyamotoi and other relapsing fever spirochetes. Cattle and sheep act as the main hosts and Rhipicephalus microplus, Haemaphysalis nepalensis, H. kolonini and Ixodes ovatus were identified as the important vector host with high prevalence or wide distribution. Only one Mus caroli (mouse) and one Sorex alpinus (shrew) were confirmed positive for relapsing fever spirochetes. Evidence of vertical transmission in ticks was also confirmed. Two known strains of B. miyamotoi and one novel relapsing fever spirochetes, B. theileri-like agent, were confirmed and described with their host adaptation, mutation, and potential risk of spreading and spillover for human beings.
UNASSIGNED: Our results provide new evidence of relapsing fever spirochetes in vector and animal hosts in Yunnan province based on large sample sizes, and offer guidance on further investigation, surveillance and monitoring of this pathogen.
UNASSIGNED: For the present study, we collected samples of ticks, wildlife, and domestic animal hosts from different counties in Yunnan province. Nucleic acids from samples were extracted, and the presence of B. miyamotoi and other relapsing fever spirochetes was confirmed using polymerase chain reaction (PCR) for the 16S rRNA specific target gene fragment. The positive samples were then amplified for partial genome of the flaB and glpQ genes. Statistical differences in its distribution were analyzed by SPSS 20 software. Sequence of partial 16S rRNA, flaB and glpQ genome were analyzed and phylogenetic trees were constructed.
UNASSIGNED: A total of 8260 samples including 2304 ticks, 4120 small mammals and 1836 blood of domestic animal hosts were collected for screening for infection of B. miyamotoi and other relapsing fever spirochetes. Cattle and sheep act as the main hosts and Rhipicephalus microplus, Haemaphysalis nepalensis, H. kolonini and Ixodes ovatus were identified as the important vector host with high prevalence or wide distribution. Only one Mus caroli (mouse) and one Sorex alpinus (shrew) were confirmed positive for relapsing fever spirochetes. Evidence of vertical transmission in ticks was also confirmed. Two known strains of B. miyamotoi and one novel relapsing fever spirochetes, B. theileri-like agent, were confirmed and described with their host adaptation, mutation, and potential risk of spreading and spillover for human beings.
UNASSIGNED: Our results provide new evidence of relapsing fever spirochetes in vector and animal hosts in Yunnan province based on large sample sizes, and offer guidance on further investigation, surveillance and monitoring of this pathogen.
摘要：
■螺旋体是一种通过硬蜱传播的螺旋体。在日本发现之后，这种病原体已经在世界各地被发现，并且越来越多地被证实是引起发热疾病的人类病原体，即复发性发热。它的存在已在中国东北得到证实。然而,在包括云南省在内的中国南部，关于米亚莫托伊和其他硬蜱传复发性螺旋体的信息很少，蜱和动物种类丰富，许多人都居住和参观娱乐。
■对于本研究，我们采集了蜱虫的样本,野生动物,和来自云南省不同县的家畜寄主。从样品中提取核酸，使用16SrRNA特异性靶基因片段的聚合酶链反应（PCR）确认了B.miyamotoi和其他复发性发热螺旋体的存在。然后扩增阳性样品的flaB和glpQ基因的部分基因组。用SPSS20软件对其分布进行统计学差异分析。部分16SrRNA的序列，分析了flaB和glpQ基因组，并构建了系统进化树。
■总共8260个样品，包括2304个蜱，收集4120只小型哺乳动物和1836只家畜宿主的血液，以筛查B.miyamotoi和其他复发性发热螺旋体的感染。牛和羊是主要寄主，而Rhipicephalusmicroplus，奈帕氏血盐，H.kolonini和Ixodesovatus被确定为重要的载体宿主，具有高患病率或广泛分布。只有一只卡罗利小母鼠（小鼠）和一只SorexAlpinus（泼妇）被证实对复发性发热螺旋体呈阳性。蜱垂直传播的证据也得到了证实。两种已知的B.miyamotoi菌株和一种新型复发性发热螺旋体，B.类似泰勒里的特工，被证实并描述为他们的宿主适应，突变,以及人类传播和溢出的潜在风险。
■我们的研究结果为云南省病媒和动物宿主中复发性发热螺旋体的大样本量提供了新的证据，并为进一步调查提供指导，监测和监测这种病原体。
■对于本研究，我们采集了蜱虫的样本,野生动物,和来自云南省不同县的家畜寄主。从样品中提取核酸，使用16SrRNA特异性靶基因片段的聚合酶链反应（PCR）确认了B.miyamotoi和其他复发性发热螺旋体的存在。然后扩增阳性样品的flaB和glpQ基因的部分基因组。用SPSS20软件对其分布进行统计学差异分析。部分16SrRNA的序列，分析了flaB和glpQ基因组，并构建了系统进化树。
■总共8260个样品，包括2304个蜱，收集4120只小型哺乳动物和1836只家畜宿主的血液，以筛查B.miyamotoi和其他复发性发热螺旋体的感染。牛和羊是主要寄主，而Rhipicephalusmicroplus，奈帕氏血盐，H.kolonini和Ixodesovatus被确定为重要的载体宿主，具有高患病率或广泛分布。只有一只卡罗利小母鼠（小鼠）和一只SorexAlpinus（泼妇）被证实对复发性发热螺旋体呈阳性。蜱垂直传播的证据也得到了证实。两种已知的B.miyamotoi菌株和一种新型复发性发热螺旋体，B.类似泰勒里的特工，被证实并描述为他们的宿主适应，突变,以及人类传播和溢出的潜在风险。
■我们的研究结果为云南省病媒和动物宿主中复发性发热螺旋体的大样本量提供了新的证据，并为进一步调查提供指导，监测和监测这种病原体。
