PDF(Pubmed)
Abstract：
Wound healing is a complex, intricate, and dynamic process that requires effective therapeutic management. The current study evaluates the wound healing potentials of methanolic extract of Cuminum cyminum L. seeds (CCS) in rats. Sprague Dawley (24) rats were distributed into four cages, wounds produced on the back of the neck, and received two daily topical treatments for 14 days: A, rats received normal saline; B, wounded rats treated with intrasite gel; C and D, rats received 0.2 mL of 250 and 500 mg/kg of CCS, respectively. After that, wound area and closure percentage were evaluated, and wound tissues were dissected for histopathological, immunohistochemical, and biochemical examinations. Acute toxicity trials of methanolic extract of CCS showed the absence of any physiological changes or mortality in rats. CCS application caused a significant reduction in wound size and a statistically elevated percentage of wound contraction than those of vehicle rats. CCS treatment caused significant up-regulation of collagen fiber, fibroblasts, and fewer inflammatory cells (inflammation) in granulation tissues. TGF-β1 (angiogenetic factor) was significantly more expressed in CCS-treated rats in comparison to normal saline-treated rats; therefore, more fibroblasts transformed into myofibroblasts (angiogenesis). CCS-treated rats showed remarkable antioxidant potentials (higher SOD and CAT enzymes) and decreased MDA (lipid peroxidation) levels in their wound tissue homogenates. Hydroxyproline amino acid (collagen) was significantly up-regulated by CCS treatment, which is commonly related to faster wound closure area. The outcomes suggest CCS as a viable new source of pharmaceuticals for wound treatment.
摘要：
伤口愈合是一个复杂的，错综复杂,和动态过程，需要有效的治疗管理。当前的研究评估了大鼠中尖锐草种子（CCS）的甲醇提取物的伤口愈合潜力。将SpragueDawley（24）大鼠分为四个笼子，颈部后部的伤口,每天接受两次局部治疗，为期14天：A，大鼠接受生理盐水;B,用石内凝胶治疗的受伤大鼠；C和D，大鼠接受了0.2mL的250和500mg/kg的CCS，分别。之后,评估伤口面积和闭合百分比，对伤口组织进行组织病理学解剖,免疫组织化学，和生化检查。CCS甲醇提取物的急性毒性试验显示大鼠没有任何生理变化或死亡率。与媒介物大鼠相比，CCS的应用导致伤口大小显着减小，并且伤口收缩的百分比在统计学上升高。CCS治疗引起胶原纤维显著上调,成纤维细胞,和较少的炎症细胞（炎症）在肉芽组织。与生理盐水治疗的大鼠相比，CCS治疗的大鼠中TGF-β1（血管生成因子）的表达明显更高；因此，更多的成纤维细胞转化为肌成纤维细胞（血管生成）。CCS处理的大鼠在其伤口组织匀浆中显示出显着的抗氧化潜力（较高的SOD和CAT酶）和降低的MDA（脂质过氧化）水平。羟脯氨酸氨基酸(胶原)被CCS处理显著上调,这通常与更快的伤口闭合区域有关。结果表明CCS是伤口治疗的可行的新药物来源。
