Abstract：
Nanoparticles (NPs) have become an important part of everyday life, including their application in dentistry. Aside from their undoubted benefits, questions regarding their risk to human health, and/or genome have arisen. However, studies concerning cytogenetic effects are completely absent. A group of women acutely exposed to an aerosol released during dental nanocomposite grinding was sampled before and after the work. Exposure monitoring including nano (PM0.1) and respirable (PM4) fractions was performed. Whole-chromosome painting for autosomes #1, #4, and gonosome X was applied to estimate the pattern of cytogenetic damage including structural and numerical alterations. The results show stable genomic frequency of translocations (FG/100), in contrast to a significant 37.8% (p<0.05) increase of numerical aberrations caused by monosomies (p<0.05), but not trisomies. Monosomies were mostly observed for chromosome X. In conclusion, exposure to nanocomposites in stomatology may lead to an increase in numerical aberrations which can be dangerous for dividing cells.
摘要：
纳米粒子(NPs)已经成为日常生活的重要组成部分,包括它们在牙科中的应用。除了他们无可置疑的好处，关于它们对人类健康的风险的问题，和/或基因组已经出现。然而,关于细胞遗传学效应的研究完全缺乏。在工作前后，对一组女性进行了采样，这些女性在牙科纳米复合材料研磨过程中急剧暴露于气溶胶中。进行包括纳米(PM0.1)和可吸入(PM4)级分的暴露监测。应用常染色体＃1，＃4和性腺X的全染色体绘画来估计细胞遗传学损伤的模式，包括结构和数值改变。结果显示易位的基因组频率稳定（FG/100），与单体引起的数值像差显著增加37.8%(p<0.05)相反(p<0.05)，但不是三体。X染色体主要观察到单体。总之，在口腔医学中暴露于纳米复合材料可能导致数值像差的增加，这对分裂的细胞可能是危险的。数据可用性声明手稿中未包含的所有数据，如原始结果，应要求提供，应发送给AR。
