Abstract：
OBJECTIVE: To assess the intraday repeatability of macular architecture measurements in glaucomatous and non-glaucomatous patients using spectral-domain optical coherence tomography (SD-OCT) and to evaluate the independence from intraindividual intraocular pressure (IOP) fluctuations.
METHODS: In this single-center, time-point comparison study, 88 eyes with glaucoma, 53 eyes with ocular hypertension (OHT), and 253 healthy eyes underwent two standardized SD-OCT and intraocular pressure (IOP) measurements on the same day with a 5-h time gap. Bland-Altman plots, intraclass correlation coefficients (ICC), and random-effects model were used to analyze repeatability of entire retinal thickness, retinal nerve fiber layer, ganglion cell layer, inner plexiform layer, and inner nuclear layer measurements.
RESULTS: Intraday measurements were highly reproducible in all 3 groups. ICC were greater than 0.90, respectively. The pairwise comparisons of morphometric parameters showed a statistically significant difference (P < 0.001, respectively) between groups (glaucoma vs. control, glaucoma vs. OHT) and a significant influence of time points. No correlation was found between IOP fluctuations and morphometric parameters (P > 0.05, respectively), except for a weak positive correlation with GCL (rho = 0.109, P = 0.031).
CONCLUSIONS: The evaluation of macular morphometric parameters of SD-OCT showed a high intraday repeatability and an excellent degree of agreement in glaucoma, ocular hypertension, and healthy groups. The fixed effects of time points were statistically significant. Except for a weak positive correlation of ganglion cell layer, variability did not appear to be affected by intraday IOP changes. Additional research is required to fully understand the impact of IOP fluctuations on macular morphometric parameters, considering the small observed IOP changes.
METHODS: In this single-center, time-point comparison study, 88 eyes with glaucoma, 53 eyes with ocular hypertension (OHT), and 253 healthy eyes underwent two standardized SD-OCT and intraocular pressure (IOP) measurements on the same day with a 5-h time gap. Bland-Altman plots, intraclass correlation coefficients (ICC), and random-effects model were used to analyze repeatability of entire retinal thickness, retinal nerve fiber layer, ganglion cell layer, inner plexiform layer, and inner nuclear layer measurements.
RESULTS: Intraday measurements were highly reproducible in all 3 groups. ICC were greater than 0.90, respectively. The pairwise comparisons of morphometric parameters showed a statistically significant difference (P < 0.001, respectively) between groups (glaucoma vs. control, glaucoma vs. OHT) and a significant influence of time points. No correlation was found between IOP fluctuations and morphometric parameters (P > 0.05, respectively), except for a weak positive correlation with GCL (rho = 0.109, P = 0.031).
CONCLUSIONS: The evaluation of macular morphometric parameters of SD-OCT showed a high intraday repeatability and an excellent degree of agreement in glaucoma, ocular hypertension, and healthy groups. The fixed effects of time points were statistically significant. Except for a weak positive correlation of ganglion cell layer, variability did not appear to be affected by intraday IOP changes. Additional research is required to fully understand the impact of IOP fluctuations on macular morphometric parameters, considering the small observed IOP changes.
摘要：
目的：使用谱域光学相干断层扫描（SD-OCT）评估青光眼和非青光眼患者黄斑结构测量的日内可重复性，并评估个体眼压（IOP）波动的独立性。
方法：在这个单中心，时间点比较研究，88眼青光眼，53只眼高眼压（OHT），253只健康眼睛在同一天进行了两次标准化的SD-OCT和眼内压(IOP)测量,时间间隔为5小时.Bland-Altman阴谋,类内相关系数(ICC)，随机效应模型用于分析整个视网膜厚度的可重复性，视网膜神经纤维层，神经节细胞层，内丛状层，和内部核层测量。
结果：3组的日内测量结果均具有很高的可重复性。ICC分别大于0.90。形态学参数的成对比较显示两组之间的统计学差异（分别为P<0.001）（青光眼与control,青光眼vs.OHT）和时间点的显着影响。眼压波动与形态测量参数无相关性(P>0.05),除与GCL呈弱正相关外(rho=0.109,P=0.031)。
结论：对SD-OCT的黄斑形态参数的评估显示出较高的日内可重复性和青光眼的良好一致性，高眼压,健康的团体。时间点的固定效应具有统计学意义。除神经节细胞层呈弱正相关外,变异性似乎不受日内眼压变化的影响。需要额外的研究来充分了解眼压波动对黄斑形态参数的影响。考虑到观察到的小眼压变化。
方法：在这个单中心，时间点比较研究，88眼青光眼，53只眼高眼压（OHT），253只健康眼睛在同一天进行了两次标准化的SD-OCT和眼内压(IOP)测量,时间间隔为5小时.Bland-Altman阴谋,类内相关系数(ICC)，随机效应模型用于分析整个视网膜厚度的可重复性，视网膜神经纤维层，神经节细胞层，内丛状层，和内部核层测量。
结果：3组的日内测量结果均具有很高的可重复性。ICC分别大于0.90。形态学参数的成对比较显示两组之间的统计学差异（分别为P<0.001）（青光眼与control,青光眼vs.OHT）和时间点的显着影响。眼压波动与形态测量参数无相关性(P>0.05),除与GCL呈弱正相关外(rho=0.109,P=0.031)。
结论：对SD-OCT的黄斑形态参数的评估显示出较高的日内可重复性和青光眼的良好一致性，高眼压,健康的团体。时间点的固定效应具有统计学意义。除神经节细胞层呈弱正相关外,变异性似乎不受日内眼压变化的影响。需要额外的研究来充分了解眼压波动对黄斑形态参数的影响。考虑到观察到的小眼压变化。
