Abstract：
This paper presents the findings of a comprehensive study exploring the synergistic effects arising from the combination of microwave ablation and pulsed electromagnetic field (PEMF) therapy on prostate cancer cells. The research encompassed five distinct experimental groups, with continuous electric field measurements conducted during the entire treatment process. Group 1 and Group 2, subjected to microwave power below 350 W, exhibited specific electric field values of 72,800 V/m and 56,600 V/m, respectively. In contrast, Group 3 and Group 4, exposed to 80 W microwave power, displayed electric field levels of approximately 1450 V/m, while remaining free from any observable electrical discharges. The migratory and invasive capacities of PC3 cells were assessed through a scratch test in all groups. Notably, cells in Group 3 and Group 4, subjected to the combined treatment of microwave ablation and PEMF, demonstrated significantly accelerated migration in comparison to those in Groups 1 and 2. Additionally, Group 5 cells, receiving PEMF treatment in isolation, exhibited decreased migratory ability. These results strongly suggest that the combined approach of microwave ablation and PEMF holds promise as a potential therapeutic intervention for prostate cancer, as it effectively reduced cell viability, induced apoptosis, and impeded migration ability in PC3 cells. Moreover, the isolated use of PEMF demonstrated potential in limiting migratory capacity, which could hold critical implications in the fight against cancer metastasis.
In this study, a new approach to treat prostate cancer by combining microwave ablation (MWA) and pulsed electromagnetic field (PEMF) therapy is explored. We used specific devices like rectangular waveguides for MWA and circular coils for PEMF. The energy sources utilized in the study comprised a magnetron tube system, similar to the microwave source found in a microwave oven, for generating microwaves, and a signal generator for producing PEMF. We used specialized equipment for MWA and PEMF to maintain controlled conditions, ensuring precise and reliable results. The research included testing various groups of prostate cancer cells exposed to different intensities of microwave power and magnetic flux density. The movement of cancer cells in different groups was examined through a wound healing assay, where cancer cells were placed on a flat surface, and we observed whether they filled the gap created by their movement. Interestingly, cells treated with both MWA and PEMF demonstrated faster movement compared to cells treated with MWA alone or PEMF alone. This combined treatment not only effectively decreased cell movement but also showed the potential cell death. The results showed that the combination of MWA and PEMF suggest a promising therapeutic strategy. The findings contribute to the development of precise and effective therapies that could enhance patient outcomes and quality of life. However, further research and validation are essential before translating these findings into clinical applications.
In this study, a new approach to treat prostate cancer by combining microwave ablation (MWA) and pulsed electromagnetic field (PEMF) therapy is explored. We used specific devices like rectangular waveguides for MWA and circular coils for PEMF. The energy sources utilized in the study comprised a magnetron tube system, similar to the microwave source found in a microwave oven, for generating microwaves, and a signal generator for producing PEMF. We used specialized equipment for MWA and PEMF to maintain controlled conditions, ensuring precise and reliable results. The research included testing various groups of prostate cancer cells exposed to different intensities of microwave power and magnetic flux density. The movement of cancer cells in different groups was examined through a wound healing assay, where cancer cells were placed on a flat surface, and we observed whether they filled the gap created by their movement. Interestingly, cells treated with both MWA and PEMF demonstrated faster movement compared to cells treated with MWA alone or PEMF alone. This combined treatment not only effectively decreased cell movement but also showed the potential cell death. The results showed that the combination of MWA and PEMF suggest a promising therapeutic strategy. The findings contribute to the development of precise and effective therapies that could enhance patient outcomes and quality of life. However, further research and validation are essential before translating these findings into clinical applications.
摘要：
本文介绍了一项综合研究的发现，探索微波消融和脉冲电磁场（PEMF）治疗对前列腺癌细胞的协同作用。这项研究包括五个不同的实验组，在整个处理过程中进行连续的电场测量。第1组和第2组，受到低于350W的微波功率，表现出72,800V/m和56,600V/m的特定电场值，分别。相比之下,第3组和第4组，暴露于80W微波功率，显示的电场水平约为1450V/m，同时保持没有任何可观察到的放电。在所有组中通过划痕试验评估PC3细胞的迁移和侵袭能力。值得注意的是,第3组和第4组的细胞接受微波消融和PEMF的联合治疗，与第1组和第2组相比，迁移速度明显加快。此外,第5组细胞,单独接受PEMF治疗，表现出迁移能力下降。这些结果强烈表明，微波消融和PEMF的联合方法有望成为前列腺癌的潜在治疗干预措施。因为它有效地降低了细胞活力，诱导细胞凋亡，并阻碍了PC3细胞的迁移能力。此外，单独使用PEMF显示出限制迁移能力的潜力，这可能对对抗癌症转移具有重要意义。
在这项研究中,探讨了微波消融(MWA)和脉冲电磁场(PEMF)联合治疗前列腺癌的新方法.我们使用特定的设备，例如MWA的矩形波导和PEMF的圆形线圈。研究中使用的能源包括磁控管系统，类似于微波炉中的微波源，用于产生微波，和用于产生PEMF的信号发生器。我们使用MWA和PEMF的专用设备来维持受控条件，确保准确可靠的结果。该研究包括测试暴露于不同强度的微波功率和磁通量密度的不同组的前列腺癌细胞。通过伤口愈合试验检查了不同组癌细胞的运动，癌细胞被放置在平坦的表面上，我们观察他们是否填补了他们运动造成的空白。有趣的是,与用单独的MWA或单独的PEMF处理的细胞相比,用MWA和PEMF处理的细胞表现出更快的运动。这种联合治疗不仅有效地减少了细胞运动，而且显示了潜在的细胞死亡。结果表明，MWA和PEMF的组合提出了有希望的治疗策略。这些发现有助于开发精确有效的治疗方法，从而提高患者的预后和生活质量。然而,在将这些发现转化为临床应用之前,进一步的研究和验证至关重要.
在这项研究中,探讨了微波消融(MWA)和脉冲电磁场(PEMF)联合治疗前列腺癌的新方法.我们使用特定的设备，例如MWA的矩形波导和PEMF的圆形线圈。研究中使用的能源包括磁控管系统，类似于微波炉中的微波源，用于产生微波，和用于产生PEMF的信号发生器。我们使用MWA和PEMF的专用设备来维持受控条件，确保准确可靠的结果。该研究包括测试暴露于不同强度的微波功率和磁通量密度的不同组的前列腺癌细胞。通过伤口愈合试验检查了不同组癌细胞的运动，癌细胞被放置在平坦的表面上，我们观察他们是否填补了他们运动造成的空白。有趣的是,与用单独的MWA或单独的PEMF处理的细胞相比,用MWA和PEMF处理的细胞表现出更快的运动。这种联合治疗不仅有效地减少了细胞运动，而且显示了潜在的细胞死亡。结果表明，MWA和PEMF的组合提出了有希望的治疗策略。这些发现有助于开发精确有效的治疗方法，从而提高患者的预后和生活质量。然而,在将这些发现转化为临床应用之前,进一步的研究和验证至关重要.
