Abstract：
In this issue, we established rapid, cost-effective, and simple detection methods including recombines polymerase amplification with lateral flow dipstick (RPA-LFD) and real-time RPA for cyprinid herpesvirus 3(CyHV-3), and evaluated their sensitivity, specificity, and applicability, the real-time RPA method could achieve sensitive diagnosis of CyHV-3 within 1.3 copies per reaction, respectively. The real-time RPA method is 10-fold more sensitive than RPA-LFD method. The exact number of CyHV-3 can be calculated in each sample by real-time RPA. The sera from koi also can be tested in these methods. In addition, no cross-reaction was observed with other related pathogens, including carp oedema virus (CEV), spring viraemia of carp virus (SVCV), cyprinid herpesvirus 1(CyHV-1), cyprinid herpesvirus 2(CyHV-2), type I grass carp reovirus (GCRV-I), type II GCRV (GCRV-II), type III GCRV (GCRV-III), and Aeromonas hydrophila.
摘要：
在这个问题上，我们迅速建立，成本效益高,和简单的检测方法，包括重组聚合酶扩增与侧流试纸（RPA-LFD）和实时RPA的鲤科疱疹病毒3（CyHV-3），并评估了它们的敏感性，特异性，和适用性，实时RPA方法可以实现每反应1.3拷贝以内的CyHV-3的灵敏诊断，分别。实时RPA方法比RPA-LFD方法灵敏度高10倍。可以通过实时RPA计算每个样品中CyHV-3的确切数量。也可以在这些方法中测试来自锦葵的血清。此外,未观察到与其他相关病原体的交叉反应，包括鲤鱼水肿病毒（CEV），鲤鱼病毒春季病毒血症（SVCV），鲤科疱疹病毒1型(CyHV-1),鲤科疱疹病毒2型(CyHV-2),I型草鱼呼肠孤病毒（GCRV-I），II型GCRV（GCRV-II），III型GCRV（GCRV-III），和嗜水气单胞菌.
