PDF(Pubmed)
Abstract：
UNASSIGNED: Clinical isolates of Acinetobacter species in South Korea are continuously exhibiting high rates of antimicrobial resistance to carbapenems, indicating that there are public health concerns among both healthcare-associated infections and community-associated infections. The aim of this study was to describe the prevalence and characteristics of carbapenem-resistant Acinetobacter isolates originating from community hospitals.
UNASSIGNED: A total of 817 non-duplicated Acinetobacter species were isolated from December 2022 to July 2023 at long-term care facilities and general hospitals in 16 regions geographically distributed throughout South Korea. Bacterial identification and antimicrobial susceptibility testing were performed using the VITEK-2 system. The bacteria were identified as Acinetobacter baumannii by blaOXA-51 PCR and as non-baumannii Acinetobacter species by rpoB sequence analysis. The carbapenem resistance genes (OXA-23, OXA-48, OXA-58, IMP, VIM, NDM, GES, and KPC) were identified via PCR and sequencing. The genetic relatedness of carbapenem-resistant A. baumannii (CRAB) isolates was assessed by multilocus sequence typing.
UNASSIGNED: A total of 659 A. baumannii and 158 non-baumannii Acinetobacter isolates, comprising 19 different species, were identified in all 16 regions. The carbapenem resistance rate was 87.4% (n=576) for the A. baumannii isolates, and all the strains produced blaOXA-23. For non-baumannii Acinetobacter, the rate of carbapenem resistance was 8.9% (n=14); this resistance was primarily caused by blaOXA-23 (n=9), followed by blaNDM-1 (n=3) and blaVIM-2 (n=2). Of the 576 CRAB isolates, clonal complex 92 (CC92) was the predominant genotypes, followed by sequence type 229 (ST229), ST373, ST397, ST447, and ST620.
UNASSIGNED: Our results showed the distribution of Acinetobacter species and showed that CC92 CRAB clinical isolates with widespread production of blaOXA-23 were predominant in community hospitals. Our findings suggest that there is a need for urgent and effective methods to reduce carbapenem resistance in A. baumannii in South Korea.
UNASSIGNED: A total of 817 non-duplicated Acinetobacter species were isolated from December 2022 to July 2023 at long-term care facilities and general hospitals in 16 regions geographically distributed throughout South Korea. Bacterial identification and antimicrobial susceptibility testing were performed using the VITEK-2 system. The bacteria were identified as Acinetobacter baumannii by blaOXA-51 PCR and as non-baumannii Acinetobacter species by rpoB sequence analysis. The carbapenem resistance genes (OXA-23, OXA-48, OXA-58, IMP, VIM, NDM, GES, and KPC) were identified via PCR and sequencing. The genetic relatedness of carbapenem-resistant A. baumannii (CRAB) isolates was assessed by multilocus sequence typing.
UNASSIGNED: A total of 659 A. baumannii and 158 non-baumannii Acinetobacter isolates, comprising 19 different species, were identified in all 16 regions. The carbapenem resistance rate was 87.4% (n=576) for the A. baumannii isolates, and all the strains produced blaOXA-23. For non-baumannii Acinetobacter, the rate of carbapenem resistance was 8.9% (n=14); this resistance was primarily caused by blaOXA-23 (n=9), followed by blaNDM-1 (n=3) and blaVIM-2 (n=2). Of the 576 CRAB isolates, clonal complex 92 (CC92) was the predominant genotypes, followed by sequence type 229 (ST229), ST373, ST397, ST447, and ST620.
UNASSIGNED: Our results showed the distribution of Acinetobacter species and showed that CC92 CRAB clinical isolates with widespread production of blaOXA-23 were predominant in community hospitals. Our findings suggest that there is a need for urgent and effective methods to reduce carbapenem resistance in A. baumannii in South Korea.
摘要：
■韩国不动杆菌的临床分离株持续表现出对碳青霉烯类抗生素的高耐药率，这表明医疗保健相关感染和社区相关感染都存在公共卫生问题。这项研究的目的是描述源自社区医院的耐碳青霉烯类不动杆菌的流行率和特征。
从2022年12月至2023年7月，在韩国16个地区的长期护理机构和综合医院共分离出817种非重复不动杆菌。使用VITEK-2系统进行细菌鉴定和抗菌药物敏感性测试。通过blaOXA-51PCR鉴定该细菌为鲍曼不动杆菌，通过rpoB序列分析鉴定为非鲍曼不动杆菌。碳青霉烯抗性基因(OXA-23，OXA-48，OXA-58，IMP，VIM,NDM,GES,和KPC）通过PCR和测序鉴定。耐碳青霉烯类鲍曼不动杆菌（CRAB）分离株的遗传相关性是通过多位点序列分型评估的。
■共659个鲍曼不动杆菌和158个非鲍曼不动杆菌分离株，由19种不同的物种组成，在所有16个地区都被确定。鲍曼不动杆菌的碳青霉烯类耐药率为87.4%(n=576),所有菌株都产生blaOXA-23。对于非鲍曼不动杆菌，碳青霉烯类耐药率为8.9%(n=14);这种耐药主要由blaOXA-23(n=9)引起,其次是blaNDM-1(n=3)和blaVIM-2(n=2)。在576个CRAB分离物中,克隆复合体92(CC92)是主要的基因型,其次是序列类型229(ST229)，ST373、ST397、ST447和ST620。
■我们的结果显示了不动杆菌种类的分布，并表明在社区医院中广泛产生blaOXA-23的CC92CRAB临床分离株占主导地位。我们的发现表明，在韩国，有必要采取紧急有效的方法来降低鲍曼不动杆菌对碳青霉烯的耐药性。
从2022年12月至2023年7月，在韩国16个地区的长期护理机构和综合医院共分离出817种非重复不动杆菌。使用VITEK-2系统进行细菌鉴定和抗菌药物敏感性测试。通过blaOXA-51PCR鉴定该细菌为鲍曼不动杆菌，通过rpoB序列分析鉴定为非鲍曼不动杆菌。碳青霉烯抗性基因(OXA-23，OXA-48，OXA-58，IMP，VIM,NDM,GES,和KPC）通过PCR和测序鉴定。耐碳青霉烯类鲍曼不动杆菌（CRAB）分离株的遗传相关性是通过多位点序列分型评估的。
■共659个鲍曼不动杆菌和158个非鲍曼不动杆菌分离株，由19种不同的物种组成，在所有16个地区都被确定。鲍曼不动杆菌的碳青霉烯类耐药率为87.4%(n=576),所有菌株都产生blaOXA-23。对于非鲍曼不动杆菌，碳青霉烯类耐药率为8.9%(n=14);这种耐药主要由blaOXA-23(n=9)引起,其次是blaNDM-1(n=3)和blaVIM-2(n=2)。在576个CRAB分离物中,克隆复合体92(CC92)是主要的基因型,其次是序列类型229(ST229)，ST373、ST397、ST447和ST620。
■我们的结果显示了不动杆菌种类的分布，并表明在社区医院中广泛产生blaOXA-23的CC92CRAB临床分离株占主导地位。我们的发现表明，在韩国，有必要采取紧急有效的方法来降低鲍曼不动杆菌对碳青霉烯的耐药性。
