PDF(Pubmed)
Abstract：
Bacillus proteolyticus MITWPUB1 is a potential producer of biosurfactants (BSs), and the organism is also found to be a producer of plant growth promoting traits, such as hydrogen cyanide and indole acetic acid (IAA), and a solubilizer of phosphate. The BSs were reportedly a blend of two classes, namely glycolipids and lipopeptides, as found by thin layer chromatography and Fourier-transform infrared spectroscopy analysis. Furthermore, semi-targeted metabolite profiling via liquid chromatography mass spectroscopy revealed the presence of phospholipids, lipopeptides, polyamines, IAA derivatives, and carotenoids. The BS showed dose-dependent antagonistic activity against Sclerotium rolfsii; scanning electron microscopy showed the effects of the BS on S. rolfsii in terms of mycelial deformations and reduced branching patterns. In vitro studies showed that the application of B. proteolyticus MITWPUB1 and its biosurfactant to seeds of Brassica juncea var local enhanced the seed germination rate. However, sawdust-carrier-based bioformulation with B. proteolyticus MITWPUB1 and its BS showed increased growth parameters for B. juncea var L. This study highlights a unique bioformulation combination that controls the growth of the phytopathogen S. rolfsii and enhances the plant growth of B. juncea var L. Bacillus proteolyticus MITWPUB1 was also shown for the first time to be a prominent BS producer with the ability to control the growth of the phytopathogen S. rolfsii.
摘要：
蛋白水解芽孢杆菌MITWPUB1是生物表面活性剂（BS）的潜在生产者，该生物也被发现是植物生长促进性状的生产者，如氰化氢和吲哚乙酸（IAA），和磷酸盐增溶剂。据报道，BS是两类的混合，即糖脂和脂肽，通过薄层色谱法和傅里叶变换红外光谱分析发现。此外，半靶向代谢物通过液相色谱质谱分析显示磷脂的存在，脂肽，多胺,IAA衍生物,和类胡萝卜素。BS对菌核菌核具有剂量依赖性拮抗活性；扫描电子显微镜显示了BS对S.rolfsii菌丝变形和减少的分支模式的影响。体外研究表明，将蛋白溶解芽孢杆菌MITWPUB1及其生物表面活性剂应用于芥菜油菜种子局部提高了种子发芽率。然而,基于木屑载体的生物制剂与蛋白水解芽孢杆菌MITWPUB1及其BS的生长参数增加。这项研究强调了一种独特的生物制剂组合，可控制植物病原体S.rolfsii的生长并增强植物的生长。蛋白水解芽孢杆菌MITWPUB1也首次被证明是一种突出的BS生产者，具有控制植物生长的能力。
