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Abstract：
This HPLC method is suitable for chitin quantitation (reported as glucosamine) in food raw materials like insects (mealworm larvae, crickets), shrimps, mushrooms and fungi in a research (non-routine) laboratory using a C18 column with HPLC system <600 bar with UV detection capability (at 265 nm). To remove interferences, the sample is defatted (Soxhlet) and deproteinized (by alkali) prior to acid hydrolysis in 6 M HCl. A five-point linear calibration (5-100 µg/mL) is used. The use of fluorescence detection (λex = 260 nm, λem = 350 nm) is also possible with this method [1].•18 min HPLC run time•LOD = 0.05 µg/mL and LOQ = 5 µg/mL.
摘要：
此HPLC方法适用于昆虫（粉虫幼虫，板球)，虾,研究（非常规）实验室中的蘑菇和真菌，使用C18色谱柱，HPLC系统<600bar，具有UV检测能力（265nm）。要消除干扰，将样品脱脂(索氏)并脱蛋白(通过碱),然后在6MHCl中进行酸水解。使用五点线性校准(5-100μg/mL)。使用荧光检测(λex=260nm，λem=350nm）也可以用这种方法[1]。•18分钟HPLC运行时间•LOD=0.05μg/mL和LOQ=5μg/mL。
