Abstract：
In this research communication we investigate the prevalence and antimicrobial susceptibility of S. aureus harboring virulent genes responsible for mastitis in cattle of Punjab, Pakistan. A total of 690 milk samples were collected from commercial dairy farms for analysis of the prevalence of subclinical and clinical mastitis and isolation of S. aureus. Virulence ability and methicillin resistance in S. aureus (MRSA) was determined by targeting the pvl (the gene for Panton-Valentine leukocidin) and mecA genes, respectively. A total of 175 S. aureus isolates exhibiting prevalence of pvl gene (6.28%) and mecA gene (22.28%) were determined. Antimicrobial susceptibility testing of pvl positive and negative MRSA against different classes of antibiotics revealed 100% resistance against β-lactams while 100% sensitivity towards tylosin and linezolid.
摘要：
在这项研究交流中，我们调查了旁遮普邦牛中携带引起乳腺炎的毒力基因的金黄色葡萄球菌的患病率和抗菌敏感性，巴基斯坦。从商业奶牛场收集了总共690个牛奶样品，用于分析亚临床和临床乳腺炎的患病率以及金黄色葡萄球菌的分离。通过靶向pvl（Panton-Valentine杀白细胞素的基因）和mecA基因来确定金黄色葡萄球菌（MRSA）的毒力能力和甲氧西林抗性，分别。测定了总共175个表现出pvl基因(6.28%)和mecA基因(22.28%)流行的金黄色葡萄球菌分离株。pvl阳性和阴性MRSA对不同类别抗生素的抗菌药物敏感性测试显示，对β-内酰胺的耐药性为100％，而对泰乐菌素和利奈唑胺的敏感性为100％。
