Abstract：
The aim of this research was to determine if there are differences in early bacterial adhesion among CAD/CAM dental materials after 24 h exposure in the oral environment. One hundred twenty specimens were prepared according to the manufacturer\'s recommendations and divided into six groups: RBC (resin-based composite), PMMA (polymethyl methacrylate), PEEK (polyether ether ketone), ZP (zirconia polished), ZG (zirconia glazed), and cobalt-chromium alloy (CoCr alloy). Twenty healthy participants were instructed to carry an intraoral device with six specimens, one per group, for 24 h. Thereafter, real-time polymerase chain reaction (qPCR) and scanning electron microscopy (SEM) analyses enabled quantification and 2D view of biofilm formed on the specimens\' surfaces. Kruskal-Wallis test and Dunn\'s post hoc analysis were used for inter-group comparison and data were presented as median (minimum-maximum). RBC specimens accumulated less bacteria, in comparison with ZG (p = 0.017) and PEEK specimens (p = 0.030), that dominated with the highest amount of adhered bacterial biofilm. PMMA, CoCr, and ZP specimens adhered more bacteria than RBC (p > 0.05), and less than ZG (p > 0.05) and PEEK (p > 0.05). The bacterial number varied considerably among participants. The obtained results enable a closer view into the susceptibility of CAD/CAM materials to microorganisms during the presence in the oral environment, which can be beneficial for a proper selection of these materials for a variety of dental restorations.
摘要：
这项研究的目的是确定在口腔环境中暴露24小时后，CAD/CAM牙科材料之间的早期细菌粘附是否存在差异。根据制造商的建议准备了一百二十个标本，分为六组：RBC（树脂基复合材料），PMMA(聚甲基丙烯酸甲酯),PEEK(聚醚醚酮),ZP（氧化锆抛光），ZG（氧化锆釉面），和钴铬合金(CoCr合金)。20名健康参与者被指示携带带有六个标本的口内装置，每组一个,24小时。此后，实时聚合酶链反应（qPCR）和扫描电子显微镜（SEM）分析使定量和二维视图的生物膜形成的标本表面。使用Kruskal-Wallis检验和Dunn的事后分析进行组间比较，数据以中位数（最小值-最大值）表示。红细胞标本积聚细菌较少，与ZG（p=0.017）和PEEK标本（p=0.030）相比，以最大量的粘附细菌生物膜为主。PMMA,CoCr,ZP标本附着的细菌多于红细胞(p>0.05)，且小于ZG(p>0.05)和PEEK(p>0.05)。参与者的细菌数量差异很大。获得的结果可以更深入地了解CAD/CAM材料在口腔环境中存在时对微生物的敏感性。这对于适当选择这些材料用于各种牙科修复是有益的。
