Abstract：
A chemically defined cryopreservation extender that maintains seminal parameters is relevant. Fifteen ejaculates from 5 stallions (n= 5; r=3) were diluted in 5 extenders: 1) EDTA-glucose based extender with egg-yolk and dimethylformamide (EY); 2) commercial equine extender (CE); 3) CE with dimethylformamide (CE-3); 4) bovine commercial extender with liposomes (OP); 5) bovine commercial extender with soybean lecithin (BIO), and frozen using a slow and a rapid temperature descent curve. Post-thaw evaluations were: sperm kinematic parameters, viability and acrosome status, membrane lipoperoxidation and DNA fragmentation. Sperm data were analysed using an ANOVA or Friedman test (results mean ± SD). Paired comparison between the two freezing curves was analysed using the Wilcoxon test. Total and progressive motility were significantly higher (P<0.05) in the EY and CE-3 samples using the slow curve, whereas for the fast curve, total and progressive motility were significantly higher (P<0.05) in the EY samples compared to all the extenders and the samples frozen in CE-3 were significantly higher than the remaining extenders (P<0.05). The percentages of live acrosome intact sperm and of live non-peroxidized sperm were significantly higher (P<0.05) in the EY extender when using either of the freezing curves and in turn, were significantly higher (P<0.05) in samples frozen in CE-3 compared to the remaining extenders. Intact DNA was significantly lower (P<0.05) in the BIO extender, using the rapid curve. To conclude, the commercial equine extender with 3% dimethylformamide, without egg-yolk, could be a suitable alternative for extenders with egg-yolk.
摘要：
保持开创性参数的化学定义的冷冻保存扩展器是相关的。将来自5种马（n=5；r=3）的15种射精剂稀释在5种补充剂中：1）基于EDTA-葡萄糖的补充剂与蛋黄和二甲基甲酰胺（EY）；2）商业马补充剂（CE）；3）CE与二甲基甲酰胺（CE-3）；4）牛商业补充剂与牛脂质体（OP）；5）商业补充剂与大豆卵磷脂（BIO），并使用缓慢而快速的温度下降曲线进行冷冻。解冻后的评估是：精子运动学参数，生存能力和顶体状态，膜脂过氧化和DNA片段化。使用ANOVA或Friedman检验分析精子数据(结果平均值±SD)。使用Wilcoxon检验分析两个冷冻曲线之间的配对比较。总运动性和进行性运动性在EY和CE-3样品中使用慢曲线显著更高(P<0.05),而对于快速曲线，与所有增量剂相比，EY样品的总运动和渐进运动均显着更高（P<0.05），在CE-3中冷冻的样品显着高于其余增量剂（P<0.05）。当使用任一冷冻曲线时，EY扩展器中活的顶体完整精子和活非过氧化精子的百分比显着升高（P<0.05），与剩余的补充剂相比，在CE-3中冷冻的样品中显著更高(P<0.05)。完整的DNA在BIO延伸剂中显著降低(P<0.05),使用快速曲线。最后，含有3%二甲基甲酰胺的商业马补充剂，没有蛋黄，可能是动物蛋白补充剂的合适替代品。
