PDF(Pubmed)
Abstract：
Honokiol is a multifunctional polyphenol present in Magnolia officinalis. The effects of honokiol as a supplement in broiler chicken diets, and the underlying mechanisms, remain unclear. Therefore, the aim of the present study was to investigate the effects of honokiol on the growth performance, antioxidant capacity, and intestinal histomorphology of broiler chickens and to explore the underlying mechanisms. In total, 240 one-day-old broilers were randomly allocated to 5 dietary treatments, with 6 replicate pens and 8 birds per pen. Birds were fed a basal diet supplemented with 0 (blank control, BC), 100, 200, or 400 mg/kg honokiol (H100, H200, and H400), or 200 mg/kg bacitracin zinc (PC) for 42 d. The results showed that H200 and H400 increased body weight gain (BWG) and decreased feed conversion ratio (FCR) during the starter period (P < 0.05). H100 and H200 increased total superoxide dismutase (T-SOD) activity in the serum and decreased malondialdehyde (MDA) amount in the jejunum on d 42 (P < 0.05). Moreover, H100 increased villus height-to-crypt depth ratio in both the jejunum and ileum on d 21 (P < 0.05). PCR analysis showed that honokiol upregulated intestinal expression of glutathione peroxidase (GSH-Px) and downregulated intestinal expression of inducible nitric oxide synthase (iNOS) on d 42 (P < 0.05). The Shannon index, which represents the microbial alpha diversity, was reduced for the PC, H200, and H400 groups. Notably, honokiol treatment altered the cecal microbial community structure and promoted the enrichment of several bacteria, including Firmicutes and Lactobacillus. Higher production of short-chain fatty acids was observed in the cecal digesta of H100 birds, accompanied by an enriched glycolysis/gluconeogenesis pathway, according to the functional prediction of the cecal microbiota. This study provides evidence that honokiol improves growth performance, antioxidant capacity, and intestinal health of broiler chickens, possibly by manipulating the composition and function of the microbial community.
摘要：
和厚朴酚是存在于厚朴中的多功能多酚。和厚朴酚在肉鸡日粮中的补充作用，以及潜在的机制，仍然不清楚。因此,本研究的目的是研究和厚朴酚对生长性能的影响，抗氧化能力，肉仔鸡的肠道组织形态及潜在机制。总的来说,240只一天大的肉鸡被随机分配到5种饮食处理，有6个复制笔和8只鸟每笔。鸟类饲喂添加0的基础日粮（空白对照，BC),100、200或400mg/kg和厚朴酚（H100、H200和H400），或200mg/kg杆菌肽锌（PC）持续42d。结果表明，H200和H400在启动期增加了体重（BWG），降低了饲料转化率（FCR）（P<0.05）。在第42天，H100和H200增加了血清中总超氧化物歧化酶（T-SOD）的活性，降低了空肠中丙二醛（MDA）的含量（P<0.05）。此外，在第21天，H100增加了空肠和回肠的绒毛高度与隐窝深度之比（P<0.05）。PCR分析显示，和厚朴酚在第42天上调肠道谷胱甘肽过氧化物酶（GSH-Px）表达，下调肠道诱导型一氧化氮合酶（iNOS）表达（P<0.05）。香农指数,代表了微生物α的多样性，为PC减少了，H200和H400组。值得注意的是,和厚朴酚处理改变了盲肠微生物群落结构，促进了几种细菌的富集，包括Firmicutes和乳酸菌。在H100鸟类的盲肠消化物中观察到较高的短链脂肪酸产量，伴随着丰富的糖酵解/糖异生途径，根据盲肠微生物群的功能预测。这项研究提供了和厚朴酚改善生长性能的证据，抗氧化能力，和肉鸡的肠道健康，可能是通过操纵微生物群落的组成和功能。
