Abstract：
Aligned with the increasing importance of bioorthogonal chemistry has been an increasing demand for more potent, affordable, multifunctional, and programmable bioorthogonal reagents. More advanced synthetic chemistry techniques, including transition-metal-catalyzed cross-coupling reactions, C-H activation, photoinduced chemistry, and continuous flow chemistry, have been employed in synthesizing novel bioorthogonal reagents for universal purposes. We discuss herein recent developments regarding the synthesis of popular bioorthogonal reagents, with a focus on s-tetrazines, 1,2,4-triazines, trans-cyclooctenes, cyclooctynes, hetero-cycloheptynes, and -trans-cycloheptenes. This review aims to summarize and discuss the most representative synthetic approaches of these reagents and their derivatives that are useful in bioorthogonal chemistry. The preparation of these molecules and their derivatives utilizes both classical approaches as well as the latest organic chemistry methodologies.
摘要：
与生物正交化学越来越重要的是对更有效的需求不断增加，负担得起的,多功能,和可编程生物正交试剂。更先进的合成化学技术，包括过渡金属催化的交叉偶联反应，C-H活化,光诱导化学，和连续流化学，已用于合成用于通用目的的新型生物正交试剂。我们在此讨论有关流行的生物正交试剂的合成的最新进展，专注于s-四嗪，1,2,4-三嗪,反式环辛烯,环辛炔,异环庚炔，和-反式-环庚烯。这篇综述旨在总结和讨论在生物正交化学中有用的这些试剂及其衍生物的最具代表性的合成方法。这些分子及其衍生物的制备利用了经典方法以及最新的有机化学方法。
