PDF(Pubmed)
Abstract：
BACKGROUND: MicroRNA-200b-3p (miR-200b-3p) plays a pivotal role in inflammatory responses and is implicated in various inflammatory disorders. In this study, we aim to explore the role of miR-200b-3p in the inflammatory response in heart failure (HF).
METHODS: Patients diagnosed with heart failure and age-matched healthy controls were studied. Peripheral blood samples from participants were collected for RNA-seq analysis to explore the expression profile of miR-200b-3p. The predictive value of miR-200b-3p and ZEB1 in the prognosis of heart failure was evaluated by analyzing the receiver operating characteristic (ROC) curve. Bioinformatics analysis and double luciferase reporter gene analysis were used to confirm the interaction between miR-200b-3p and ZEB1. Real-time quantitative polymerase chain reaction (QRT-PCR) was used to detect the expression levels of miR-200b-3p and ZEB1 in cardiopulmonary bypass. Additionally, the effects of miR-200b-3p on myocardial cell line (H9c2) injury were evaluated by enzyme-linked immunosorbent assay (ELISA).
RESULTS: In the extracardiac circulation of HF patients, miR-200b-3p expression was significantly reduced, while ZEB1 levels were notably elevated. Analysis of the ROC curve revealed that miR-200b-3p and ZEB1 have predictive value in the prognosis of HF patients. The double luciferase reporter experiment demonstrated that miR-200b-3p binds to ZEB1 and inhibits its expression. Overexpression of miR-200b-3p demonstrated a remarkable ability to alleviate inflammation and inhibit the damage to myocardial cells in vivo.
CONCLUSIONS: MiR-200b-3p can target and inhibit ZEB1, reducing the inflammatory reaction of myocardial cells. The miR-200b-3p/ZEB1 network may be helpful in preventing and treating HF.
METHODS: Patients diagnosed with heart failure and age-matched healthy controls were studied. Peripheral blood samples from participants were collected for RNA-seq analysis to explore the expression profile of miR-200b-3p. The predictive value of miR-200b-3p and ZEB1 in the prognosis of heart failure was evaluated by analyzing the receiver operating characteristic (ROC) curve. Bioinformatics analysis and double luciferase reporter gene analysis were used to confirm the interaction between miR-200b-3p and ZEB1. Real-time quantitative polymerase chain reaction (QRT-PCR) was used to detect the expression levels of miR-200b-3p and ZEB1 in cardiopulmonary bypass. Additionally, the effects of miR-200b-3p on myocardial cell line (H9c2) injury were evaluated by enzyme-linked immunosorbent assay (ELISA).
RESULTS: In the extracardiac circulation of HF patients, miR-200b-3p expression was significantly reduced, while ZEB1 levels were notably elevated. Analysis of the ROC curve revealed that miR-200b-3p and ZEB1 have predictive value in the prognosis of HF patients. The double luciferase reporter experiment demonstrated that miR-200b-3p binds to ZEB1 and inhibits its expression. Overexpression of miR-200b-3p demonstrated a remarkable ability to alleviate inflammation and inhibit the damage to myocardial cells in vivo.
CONCLUSIONS: MiR-200b-3p can target and inhibit ZEB1, reducing the inflammatory reaction of myocardial cells. The miR-200b-3p/ZEB1 network may be helpful in preventing and treating HF.
摘要：
背景：MicroRNA-200b-3p（miR-200b-3p）在炎症反应中起关键作用，并且与各种炎症障碍有关。在这项研究中,我们旨在探讨miR-200b-3p在心力衰竭（HF）炎症反应中的作用。
方法：研究了诊断为心力衰竭的患者和年龄匹配的健康对照。收集来自参与者的外周血样品用于RNA-seq分析以探索miR-200b-3p的表达谱。通过分析受试者工作特征(ROC)曲线评价miR-200b-3p和ZEB1对心力衰竭预后的预测价值。生物信息学分析和双荧光素酶报告基因分析用于证实miR-200b-3p与ZEB1之间的相互作用。实时定量聚合酶链反应(QRT-PCR)检测miR-200b-3p和ZEB1在体外循环中的表达水平。此外,通过酶联免疫吸附试验（ELISA）评估miR-200b-3p对心肌细胞系（H9c2）损伤的影响。
结果：在HF患者的心外循环中，miR-200b-3p表达显著降低,而ZEB1水平显著升高。ROC曲线分析表明miR-200b-3p和ZEB1对HF患者的预后具有预测价值。双荧光素酶报告基因实验证明miR-200b-3p与ZEB1结合并抑制其表达。miR-200b-3p的过表达在体内表现出显著的减轻炎症和抑制心肌细胞损伤的能力。
结论：MiR-200b-3p可靶向抑制ZEB1，减轻心肌细胞的炎症反应。miR-200b-3p/ZEB1网络可能有助于预防和治疗HF。
方法：研究了诊断为心力衰竭的患者和年龄匹配的健康对照。收集来自参与者的外周血样品用于RNA-seq分析以探索miR-200b-3p的表达谱。通过分析受试者工作特征(ROC)曲线评价miR-200b-3p和ZEB1对心力衰竭预后的预测价值。生物信息学分析和双荧光素酶报告基因分析用于证实miR-200b-3p与ZEB1之间的相互作用。实时定量聚合酶链反应(QRT-PCR)检测miR-200b-3p和ZEB1在体外循环中的表达水平。此外,通过酶联免疫吸附试验（ELISA）评估miR-200b-3p对心肌细胞系（H9c2）损伤的影响。
结果：在HF患者的心外循环中，miR-200b-3p表达显著降低,而ZEB1水平显著升高。ROC曲线分析表明miR-200b-3p和ZEB1对HF患者的预后具有预测价值。双荧光素酶报告基因实验证明miR-200b-3p与ZEB1结合并抑制其表达。miR-200b-3p的过表达在体内表现出显著的减轻炎症和抑制心肌细胞损伤的能力。
结论：MiR-200b-3p可靶向抑制ZEB1，减轻心肌细胞的炎症反应。miR-200b-3p/ZEB1网络可能有助于预防和治疗HF。
