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Abstract：
OBJECTIVE: To investigate the associations of Insulin-like growth factor-II (IGF2) gene, Insulin-like growth factor-II receptor (IGF2R) gene and Insulin-like growth factor-II binding protein 2 (IGF2BP2) gene polymorphisms with the susceptibility to gestational diabetes mellitus (GDM) in Chinese population.
METHODS: A total of 1703 pregnant women (835 GDM and 868 Non-GDM) were recruited in this case-control study. All participants underwent prenatal 75 g oral glucose tolerance test (OGTT) examinations during 24-28 gestational weeks at the Maternal and Child Health Hospital of Hubei Province from January 15, 2018 to March 31, 2019. Genotyping of candidate SNPs (IGF2 rs680, IGF2R rs416572, IGF2BP2 rs4402960, rs1470579, rs1374910, rs11705701, rs6777038, rs16860234, rs7651090) was performed on Sequenom MassARRAY platform. Logistic regression analysis was conducted to investigate the associations between candidate SNPs and risk of GDM. In addition, multifactor dimensionality reduction (MDR) method was applied to explore the effects of gene-gene interactions on GDM risk.
RESULTS: There were significant distribution differences between GDM group and non-GDM group in age, pre-pregnancy BMI, education level and family history of diabetes (P < 0.05). After adjusted for age, pre-pregnancy BMI, education level and family history of diabetes, there were no significant associations of the candidate SNPs polymorphisms and GDM risk (P > 0.05). Furthermore, there were no gene-gene interactions on the GDM risk among the candidate SNPs (P > 0.05). However, the fasting blood glucose (FBG) levels of rs6777038 CT carriers were significantly lower than TT carriers (4.69±0.69 vs. 5.03±1.57 mmol/L, P < 0.01), and the OGTT-2h levels of rs6777038 CC and CT genotype carriers were significantly lower than TT genotype carriers (8.10±1.91 and 8.08±1.87 vs. 8.99±2.90 mmol/L, P < 0.01).
CONCLUSIONS: IGF2 rs680, IGF2R rs416572, IGF2BP2 rs4402960, rs1470579, rs11705701, rs6777038, rs16860234, rs7651090 polymorphisms were not significantly associated with GDM risk in Wuhan, China. Further lager multicenter researches are needed to confirm these results.
METHODS: A total of 1703 pregnant women (835 GDM and 868 Non-GDM) were recruited in this case-control study. All participants underwent prenatal 75 g oral glucose tolerance test (OGTT) examinations during 24-28 gestational weeks at the Maternal and Child Health Hospital of Hubei Province from January 15, 2018 to March 31, 2019. Genotyping of candidate SNPs (IGF2 rs680, IGF2R rs416572, IGF2BP2 rs4402960, rs1470579, rs1374910, rs11705701, rs6777038, rs16860234, rs7651090) was performed on Sequenom MassARRAY platform. Logistic regression analysis was conducted to investigate the associations between candidate SNPs and risk of GDM. In addition, multifactor dimensionality reduction (MDR) method was applied to explore the effects of gene-gene interactions on GDM risk.
RESULTS: There were significant distribution differences between GDM group and non-GDM group in age, pre-pregnancy BMI, education level and family history of diabetes (P < 0.05). After adjusted for age, pre-pregnancy BMI, education level and family history of diabetes, there were no significant associations of the candidate SNPs polymorphisms and GDM risk (P > 0.05). Furthermore, there were no gene-gene interactions on the GDM risk among the candidate SNPs (P > 0.05). However, the fasting blood glucose (FBG) levels of rs6777038 CT carriers were significantly lower than TT carriers (4.69±0.69 vs. 5.03±1.57 mmol/L, P < 0.01), and the OGTT-2h levels of rs6777038 CC and CT genotype carriers were significantly lower than TT genotype carriers (8.10±1.91 and 8.08±1.87 vs. 8.99±2.90 mmol/L, P < 0.01).
CONCLUSIONS: IGF2 rs680, IGF2R rs416572, IGF2BP2 rs4402960, rs1470579, rs11705701, rs6777038, rs16860234, rs7651090 polymorphisms were not significantly associated with GDM risk in Wuhan, China. Further lager multicenter researches are needed to confirm these results.
摘要：
目的：探讨胰岛素样生长因子-II(IGF2)基因的相关性,胰岛素样生长因子-II受体(IGF2R)基因和胰岛素样生长因子-II结合蛋白2(IGF2BP2)基因多态性与中国人群妊娠期糖尿病(GDM)易感性的关系.
方法：本病例对照研究共招募了1703名孕妇（835名GDM和868名非GDM）。所有参与者于2018年1月15日至2019年3月31日在湖北省妇幼保健院24-28周进行产前75g口服葡萄糖耐量试验(OGTT)检查。在SequenomMassARRAY平台上进行候选SNP(IGF2rs680,IGF2Rrs416572,IGF2BP2rs4402960,rs1470579,rs1374910,rs11705701,rs6777038,rs16860234,rs7651090)的基因分型。进行Logistic回归分析以探讨候选SNP与GDM风险之间的关系。此外,采用多因素降维（MDR）方法探讨基因-基因相互作用对GDM风险的影响。
结果：GDM组和非GDM组的年龄分布有显著差异，孕前BMI,文化程度及糖尿病家族史(P<0.05)。在调整了年龄后，孕前BMI,教育水平和糖尿病家族史，候选SNPs多态性与GDM风险无显著相关性(P>0.05)。此外，候选SNPs中GDM风险无基因-基因交互作用(P>0.05)。然而,rs6777038CT携带者的空腹血糖（FBG）水平明显低于TT携带者（4.69±0.69vs.5.03±1.57mmol/L，P<0.01),rs6777038CC和CT基因型携带者的OGTT-2h水平明显低于TT基因型携带者（8.10±1.91和8.08±1.87vs.8.99±2.90mmol/L,P<0.01)。
结论：IGF2rs680，IGF2Rrs416572，IGF2BP2rs4402960，rs1470579，rs11705701，rs6777038，rs16860234，rs7651090多态性与武汉地区GDM风险无显著相关性，中国。需要进一步的多中心研究来证实这些结果。
方法：本病例对照研究共招募了1703名孕妇（835名GDM和868名非GDM）。所有参与者于2018年1月15日至2019年3月31日在湖北省妇幼保健院24-28周进行产前75g口服葡萄糖耐量试验(OGTT)检查。在SequenomMassARRAY平台上进行候选SNP(IGF2rs680,IGF2Rrs416572,IGF2BP2rs4402960,rs1470579,rs1374910,rs11705701,rs6777038,rs16860234,rs7651090)的基因分型。进行Logistic回归分析以探讨候选SNP与GDM风险之间的关系。此外,采用多因素降维（MDR）方法探讨基因-基因相互作用对GDM风险的影响。
结果：GDM组和非GDM组的年龄分布有显著差异，孕前BMI,文化程度及糖尿病家族史(P<0.05)。在调整了年龄后，孕前BMI,教育水平和糖尿病家族史，候选SNPs多态性与GDM风险无显著相关性(P>0.05)。此外，候选SNPs中GDM风险无基因-基因交互作用(P>0.05)。然而,rs6777038CT携带者的空腹血糖（FBG）水平明显低于TT携带者（4.69±0.69vs.5.03±1.57mmol/L，P<0.01),rs6777038CC和CT基因型携带者的OGTT-2h水平明显低于TT基因型携带者（8.10±1.91和8.08±1.87vs.8.99±2.90mmol/L,P<0.01)。
结论：IGF2rs680，IGF2Rrs416572，IGF2BP2rs4402960，rs1470579，rs11705701，rs6777038，rs16860234，rs7651090多态性与武汉地区GDM风险无显著相关性，中国。需要进一步的多中心研究来证实这些结果。
