PDF(Pubmed)
Abstract：
The aim of this study is to explore the pharmacological properties of the essential oil derived from Ptychotis verticillata Duby (PVEO), a medicinal plant native to Morocco, focusing on its antidiabetic, anti-tyrosinase, and anti-inflammatory effects. Additionally, the study aims to characterize the phytochemical composition of PVEO and evaluate its potential as a natural therapeutic alternative for various health conditions. To achieve this, phytochemical analysis was conducted using gas chromatography-mass spectrometry (GC-MS). Furthermore, in vitro assessments were conducted to investigate PVEO\'s antidiabetic activity by inhibiting α-amylase, xanthine oxidase, and α-glucosidase. Tests were also undertaken to evaluate the anti-inflammatory effect of PVEO on RAW 264.7 cells stimulated by lipopolysaccharide (LPS), as well as its efficacy as an anti-tyrosinase agent and its lipoxygenase inhibition activity. The results of the phytochemical analysis revealed that PVEO is rich in terpene compounds, with percentages of 40.35 % γ-terpinene, 22.40 % carvacrol, and 19.77 % β-cymene. Moreover, in vitro evaluations demonstrated that PVEO exhibits significant inhibitory activity against α-amylase, xanthine oxidase, and α-glucosidase, indicating promising antidiabetic, and anti-gout potential. Furthermore, PVEO showed significant anti-tyrosinase activity, with an IC50 of 27.39 ± 0.44 μg/mL, and remarkable lipoxygenase inhibition (87.33 ± 2.6 %), suggesting its candidacy for dermatoprotection. Additionally, PVEO displayed a dose-dependent capacity to attenuate the production of NO and PGE2, two inflammatory mediators implicated in various pathologies, without compromising cellular viability. The findings of this study provide a solid foundation for future research on natural therapies and the development of new drugs, highlighting the therapeutic potential of PVEO in the treatment of gout, diabetes, pigmentation disorders, and inflammation.
摘要：
这项研究的目的是探索从Duby（PVEO）中提取的精油的药理特性，一种原产于摩洛哥的药用植物，专注于它的抗糖尿病药，抗酪氨酸酶,和抗炎作用。此外,该研究旨在表征PVEO的植物化学成分，并评估其作为各种健康状况的天然治疗替代品的潜力。为了实现这一点，使用气相色谱-质谱（GC-MS）进行植物化学分析。此外，进行体外评估以研究PVEO通过抑制α-淀粉酶的抗糖尿病活性，黄嘌呤氧化酶,和α-葡萄糖苷酶。还进行了测试以评估PVEO对脂多糖（LPS）刺激的RAW264.7细胞的抗炎作用。以及其作为抗酪氨酸酶剂的功效及其脂氧合酶抑制活性。植物化学分析结果表明，PVEO富含萜烯化合物，γ-萜品烯的百分比为40.35％，22.40%香芹酚,和19.77%的β-异丙基苯。此外，体外评价表明，PVEO对α-淀粉酶具有显著的抑制活性，黄嘌呤氧化酶,和α-葡萄糖苷酶，表明有希望的抗糖尿病药，和抗痛风的潜力。此外，PVEO显示出显著的抗酪氨酸酶活性,IC50为27.39±0.44μg/mL，和显着的脂氧合酶抑制（87.33±2.6％），表明它是皮肤保护的候选人。此外,PVEO显示出剂量依赖性的减弱NO和PGE2的产生的能力，这两种炎症介质与各种病理有关。不损害细胞活力。这项研究的发现为未来自然疗法的研究和新药的开发提供了坚实的基础,强调PVEO在痛风治疗中的治疗潜力，糖尿病,色素沉着症,和炎症。
