PDF(Pubmed)
Abstract：
DNA is commonly employed as a substrate for the building of artificial logic networks due to its excellent biocompatibility and programmability. Till now, DNA logic circuits are rapidly evolving to accomplish advanced operations. Nonetheless, nowadays, most DNA circuits remain to be disposable and lack of field programmability and thereby limits their practicability. Herein, inspired by the Configurable Logic Block (CLB), the CLB-based erasable field-programmable DNA circuit that uses clip strands as its operation-controlling signals is presented. It enables users to realize diverse functions with limited hardware. CLB-based basic logic gates (OR and AND) are first constructed and demonstrated their erasability and field programmability. Furthermore, by adding the appropriate operation-controlling strands, multiple rounds of programming are achieved among five different logic operations on a two-layer circuit. Subsequently, a circuit is successfully built to implement two fundamental binary calculators: half-adder and half-subtractor, proving that the design can imitate silicon-based binary circuits. Finally, a comprehensive CLB-based circuit is built that enables multiple rounds of switch among seven different logic operations including half-adding and half-subtracting. Overall, the CLB-based erasable field-programmable circuit immensely enhances their practicability. It is believed that design can be widely used in DNA logic networks due to its efficiency and convenience.
摘要：
由于其优异的生物相容性和可编程性,DNA通常被用作构建人工逻辑网络的基底。到现在为止,DNA逻辑电路正在迅速发展以完成高级操作。尽管如此，如今,大多数DNA电路仍然是一次性的，缺乏现场可编程性，从而限制了它们的实用性。在这里，受可配置逻辑块(CLB)的启发，提出了基于CLB的可擦除现场可编程DNA电路，该电路使用夹线作为其操作控制信号。它使用户能够以有限的硬件实现不同的功能。首先构造基于CLB的基本逻辑门（OR和AND），并证明了它们的可擦除性和场可编程性。此外，通过添加适当的操作控制链，在两层电路上的五个不同的逻辑运算之间实现多轮编程。随后，成功构建了一个电路来实现两个基本的二进制计算器：半加器和半减法器，证明了该设计可以模仿硅基二进制电路。最后,建立了一个全面的基于CLB的电路，可以在七个不同的逻辑运算中进行多轮切换，包括半加法和半减法。总的来说,基于CLB的可擦除现场可编程电路极大地增强了其实用性。人们认为，由于其效率和便利性，设计可以广泛应用于DNA逻辑网络中。
