PDF(Pubmed)
Abstract：
Chagas disease, caused by the protozoa Trypanosoma cruzi, continues to be a serious public health problem in Latin America, worsened by the limitations in its detection. Given the importance of developing new diagnostic methods for this disease, the present review aimed to verify the number of publications dedicated to research on peptides that demonstrate their usefulness in serodiagnosis. To this end, a bibliographic survey was conducted on the PubMed platform using the keyword \"peptide\" or \"epitope\" combined with \"Chagas disease\" or \"Trypanosoma cruzi\"; \"diagno*\" or \"serodiagnosis\" or \"immunodiagnosis\", without period restriction. An increasing number of publications on studies employing peptides in ELISA and rapid tests assays was verified, which confirms the expansion of research in this field. It is possible to observe that many of the peptides tested so far originate from proteins widely used in the diagnosis of Chagas, and many of them are part of commercial tests developed. In this sense, as expected, promising results were obtained for several peptides when tested in ELISA, as many of them exhibited sensitivity and specificity values above 90%. Furthermore, some peptides have been tested in several studies, confirming their diagnostic potential. Despite the promising results observed, it is possible to emphasize the need for extensive testing of peptides, using different serological panels, in order to confirm their potential. The importance of producing an effective assay capable of detecting the clinical stages of the disease, as well as new immunogenic antigens that enable new serological diagnostic tools for Chagas disease, is evident.
摘要：
查加斯病,由原生动物克氏锥虫引起的，在拉丁美洲仍然是一个严重的公共卫生问题，由于其检测的局限性而恶化。鉴于开发这种疾病的新诊断方法的重要性，本综述旨在验证致力于肽研究的出版物数量，这些出版物证明了它们在血清诊断中的有用性。为此,在PubMed平台上使用关键词“肽”或“表位”与“恰加斯病”或“克氏锥虫”相结合进行了书目调查；“diagno*”或“血清诊断”或“免疫诊断”，没有期限限制。关于在ELISA和快速测试测定中使用肽的研究的出版物越来越多，得到了验证。这证实了该领域研究的扩展。可以观察到，迄今为止测试的许多肽都来自广泛用于Chagas诊断的蛋白质，其中许多是商业测试的一部分。在这个意义上,正如预期的那样，在ELISA中测试时，几种肽获得了有希望的结果，因为他们中的许多表现出超过90％的敏感性和特异性值。此外，一些肽已经在几项研究中进行了测试，确认其诊断潜力。尽管观察到了有希望的结果，有可能强调需要对肽进行广泛的测试，使用不同的血清学面板，以确认他们的潜力。产生能够检测疾病临床分期的有效测定法的重要性，以及新的免疫原性抗原，这些抗原能够为恰加斯病提供新的血清学诊断工具，很明显。
