Abstract：
Osteoporosis is a prevalent condition characterized by bone loss and decreased skeletal strength, resulting in an elevated risk of fractures. Calcium plays a crucial role in preventing and managing osteoporosis. However, traditional calcium supplements have limited bioavailability, poor solubility, and adverse effects. In this study, we isolated a natural soluble biopolymer, calcium polymalate (PMACa), from the fermentation broth of the fungus Aureobasidium pullulans, to investigate its potential as an anti-osteoporosis therapeutic agent. Characterization revealed that linear PMA-Ca chains juxtaposed to form a porous, rod-like state, in the presence of Ca2+. In vivo mouse models demonstrated that PMA-Ca significantly promoted the conversion of serum calcium into bone calcium, and stimulated bone growth and osteogenesis. Additionally, PMA-Ca alleviated exercise fatigue in mice by facilitating the removal of essential metabolites, such as serum lactate (BLA) and blood urea nitrogen (BUN), from their bloodstream. In vitro studies further showed that PMA-Ca strengthened osteoblast cell activity, proliferation, and mineralization. And PMA-Ca upregulated the expression of some genes involved in osteoblast differentiation, indicating a potential correlation between bone formation and PMACa. These findings indicate that soluble PMA-Ca has the potential to be a novel biopolymer-based calcium supplement with sustainable production sourced from the fermentation industry.
摘要：
骨质疏松症是一种普遍存在的疾病，其特征是骨质流失和骨骼强度下降。导致骨折的风险增加。钙在预防和管理骨质疏松症中起着至关重要的作用。然而,传统的钙补充剂生物利用度有限，溶解性差，和不利影响。在这项研究中,我们分离出一种天然的可溶性生物聚合物，多苹果酸钙（PMCa），从真菌出芽梭菌的发酵液中提取，研究其作为抗骨质疏松治疗剂的潜力。表征表明，线性PMA-Ca链并列形成多孔，杆状状态,在Ca2+的存在下。体内小鼠模型表明，PMA-Ca显着促进血清钙转化为骨钙，并刺激骨骼生长和成骨。此外,PMA-Ca通过促进必需代谢物的去除来缓解小鼠的运动疲劳，如血清乳酸（BLA）和血尿素氮（BUN），从他们的血液。体外研究进一步表明，PMA-Ca增强成骨细胞活性，扩散,和矿化。PMA-Ca上调成骨细胞分化相关基因的表达,表明骨形成与PMCa之间存在潜在的相关性。这些发现表明可溶性PMA-Ca具有成为新型的基于生物聚合物的钙补充剂的潜力,其具有源自发酵工业的可持续生产。
