UNASSIGNED: After establishing rat OTM models, LIPUS was used to stimulate rats locally. OTM distance and alveolar bone density were assessed using micro-computed tomography, and histological analyses included hematoxylin and eosin staining, tartrate-resistant acid phosphatase staining and immunohistochemical staining. GsMTx4 and Yoda1 were respectively utilized for Piezo1 functional inhibition and activation experiments in rats. We isolated human PDLCs (hPDLCs) in vitro and evaluated the effects of LIPUS on the osteogenic differentiation of force-treated hPDLCs using real-time quantitative PCR, Western blot, alkaline phosphatase and alizarin red staining. Small interfering RNA and Yoda1 were employed to validate the role of Piezo1 in this process.
UNASSIGNED: LIPUS promoted osteoclast differentiation and accelerated OTM in rats. Furthermore, LIPUS alleviated alveolar bone resorption under pressure and enhanced osteogenesis of force-treated PDLCs both in vivo and in vitro by downregulating Piezo1 expression. Subsequent administration of GsMTx4 in rats and siPIEZO1 transfection in hPDLCs attenuated the inhibitory effect on osteogenic differentiation under pressure, whereas LIPUS efficacy was partially mitigated. Yoda1 treatment inhibited osteogenic differentiation of hPDLCs, resulting in reduced expression of Collagen Ⅰα1 and osteocalcin in the periodontal ligament. However, LIPUS administration was able to counteract these effects.
UNASSIGNED: This research unveils that LIPUS promotes the osteogenesis of force-treated PDLCs via downregulating Piezo1.
■建立大鼠OTM模型后,LIPUS用于局部刺激大鼠。使用显微计算机断层扫描评估OTM距离和牙槽骨密度,组织学分析包括苏木精和伊红染色,抗酒石酸酸性磷酸酶染色和免疫组织化学染色。GsMTx4和Yoda1分别用于大鼠的Piezo1功能抑制和激活实验。我们在体外分离了人PDLCs(hPDLCs),并使用实时定量PCR评估了LIPUS对力处理的hPDLCs成骨分化的影响,蛋白质印迹,碱性磷酸酶和茜素红染色。使用小干扰RNA和Yoda1来验证Piezo1在此过程中的作用。
■LIPUS促进大鼠破骨细胞分化并加速OTM。此外,LIPUS通过下调Piezo1表达在体内和体外减轻压力下的牙槽骨吸收并增强力处理的PDLCs的成骨作用。随后在大鼠中施用GsMTx4和在hPDLCs中的siPIEZO1转染减弱了在压力下对成骨分化的抑制作用,而LIPUS疗效部分减轻。Yoda1处理抑制hPDLCs成骨分化,导致牙周膜中Ⅰ型胶原α1和骨钙蛋白的表达降低。然而,LIPUS管理能够抵消这些影响。
■这项研究揭示了LIPUS通过下调Piezo1促进力处理PDLCs的成骨作用。