PDF(Pubmed)
Abstract：
The sea cucumber (H. glaberrima) is a species found in the shallow waters near coral reefs and seagrass beds in Puerto Rico. To characterize the microbial taxonomic composition and functional profiles present in the sea cucumber, total DNA was obtained from their intestinal system, fosmid libraries constructed, and subsequent sequencing was performed. The diversity profile displayed that the most predominant domain was Bacteria (76.56 %), followed by Viruses (23.24 %) and Archaea (0.04 %). Within the 11 phyla identified, the most abundant was Proteobacteria (73.16 %), followed by Terrabacteria group (3.20 %) and Fibrobacterota, Chlorobiota, Bacteroidota (FCB) superphylum (1.02 %). The most abundant species were Porvidencia rettgeri (21.77 %), Pseudomonas stutzeri (14.78 %), and Alcaligenes faecalis (5.00 %). The functional profile revealed that the most abundant functions are related to transporters, MISC (miscellaneous information systems), organic nitrogen, energy, and carbon utilization. The data collected in this project on the diversity and functional profiles of the intestinal system of the H. glaberrima provided a detailed view of its microbial ecology. These findings may motivate comparative studies aimed at understanding the role of the microbiome in intestinal regeneration.
摘要：
海参（H.glaberrima）是在波多黎各珊瑚礁和海草床附近的浅水区发现的一种物种。为了表征海参中存在的微生物分类组成和功能概况，从他们的肠道系统获得总DNA，构建的Fosmid库，随后进行测序。多样性谱显示，最主要的结构域是细菌(76.56%)，其次是病毒（23.24%）和古生菌（0.04%）。在确定的11个门中，最丰富的是变形杆菌(73.16%)，其次是Terrabacteria组（3.20%）和纤维杆菌，叶绿素,类杆菌(FCB)超门(1.02%)。最丰富的物种是Porvidenciarettgeri（21.77％），猪油假单胞菌(14.78%),和粪产碱菌(5.00%)。功能概况表明，最丰富的功能与转运蛋白有关，MISC(杂项信息系统)，有机氮,能源,和碳利用。在该项目中收集的有关H.glaberrima肠道系统的多样性和功能概况的数据提供了其微生物生态学的详细视图。这些发现可能会激发旨在了解微生物组在肠道再生中的作用的比较研究。
