OBJECTIVE: To identify the characteristic gut genera in obese and normal-weight children (8-12-year-old) using 16S rDNA sequencing, and provide a basis for subsequent mechanistic studies and prevention strategies for childhood obesity.
METHODS: Thirty each normal-weight, 1:1 matched for age and sex, and obese children, with an obese status from 2020 to 2022, were included in the control and obese groups, respectively. Basic information was collected through questionnaires and body measurements were obtained from both obese and normal-weight children. Fecal samples were collected from both groups and subjected to 16S rDNA sequencing using an Illumina MiSeq sequencing platform for gut microbiota diversity analysis.
RESULTS: Significant differences in BMI and body-fat percentage were observed between the two groups. The Ace and Chao1 indices were significantly lower in the obese group than those in the control group, whereas differences were not significant in the Shannon and Simpson indices. Kruskal-Wallis tests indicated significant differences in unweighted and weighted UniFrac distances between the gut microbiota of normal-weight and obese children (P < 0.01), suggesting substantial disparities in both the species and quantity of gut microbiota between the two groups. Prevotella, Firmicutes, Bacteroides, and Sanguibacteroides were more abundant in the obese and control groups, respectively. Heatmap results demonstrated significant differences in the gut microbiota composition between obese and normal-weight children.
CONCLUSIONS: Obese children exhibited lower α-diversity in their gut microbiota than did the normal-weight children. Significant differences were observed in the composition of gut microbiota between obese and normal-weight children.
目的:使用16SrDNA测序技术鉴定肥胖和正常体重儿童(8-12岁)的特征性肠属,并为后续儿童肥胖的机制研究和预防策略提供依据。
方法:每个正常体重30,1:1匹配的年龄和性别,肥胖儿童,从2020年到2022年为肥胖状态,被纳入对照组和肥胖组,分别。通过问卷调查收集基本信息,并从肥胖和正常体重儿童获得身体测量值。从两组收集粪便样品并使用IlluminaMiSeq测序平台进行16SrDNA测序以进行肠道微生物群多样性分析。
结果:两组的BMI和体脂百分比存在显著差异。肥胖组Ace和Chao1指数明显低于对照组,而Shannon和Simpson指数差异不显著。Kruskal-Wallis检验表明,正常体重和肥胖儿童的肠道微生物群之间的未加权和加权UniFrac距离存在显着差异(P<0.01)。这表明两组之间肠道微生物群的种类和数量存在巨大差异。普雷沃氏菌,Firmicutes,拟杆菌,在肥胖组和对照组中,杆菌属的含量更高,分别。热图结果显示,肥胖儿童和正常体重儿童之间的肠道微生物群组成存在显着差异。
结论:肥胖儿童的肠道菌群表现出比正常体重儿童低的α-多样性。肥胖和正常体重儿童的肠道菌群组成存在显着差异。