PDF(Pubmed)
Abstract：
The reconstruction of a stable, nipple-shaped cartilage graft that precisely matches the natural nipple in shape and size on the contralateral side is a clinical challenge. While 3D printing technology can efficiently and accurately manufacture customized complex structures, it faces limitations due to inadequate blood supply, which hampers the stability of nipple-shaped cartilage grafts produced using this technology. To address this issue, we employed a biodegradable biomaterial, Poly(lactic-co-glycolic acid) (PLGA), loaded with Cell-Free Fat Extract (Ceffe). Ceffe has demonstrated the ability to promote angiogenesis and cell proliferation, making it an ideal bio-ink for bioprinting precise nipple-shaped cartilage grafts. We utilized the Ceffe/PLGA scaffold to create a porous structure with a precise nipple shape. This scaffold exhibited favorable porosity and pore size, ensuring stable shape maintenance and satisfactory biomechanical properties. Importantly, it could release Ceffe in a sustained manner. Our in vitro results confirmed the scaffold\'s good biocompatibility and its ability to promote angiogenesis, as evidenced by supporting chondrocyte proliferation and endothelial cell migration and tube formation. Furthermore, after 8 weeks of in vivo culture, the Ceffe/PLGA scaffold seeded with chondrocytes regenerated into a cartilage support structure with a precise nipple shape. Compared to the pure PLGA group, the Ceffe/PLGA scaffold showed remarkable vascular formation, highlighting the beneficial effects of Ceffe. These findings suggest that our designed Ceffe/PLGA scaffold with a nipple shape represents a promising strategy for precise nipple-shaped cartilage regeneration, laying a foundation for subsequent nipple reconstruction.
摘要：
一个稳定的重建，乳头状软骨移植物在对侧的形状和大小上与天然乳头精确匹配是一项临床挑战。虽然3D打印技术可以高效准确地制造定制的复杂结构，由于血液供应不足，它面临着限制，这阻碍了使用这种技术生产的乳头状软骨移植物的稳定性。为了解决这个问题，我们使用了一种可生物降解的生物材料，聚乳酸-乙醇酸共聚物(PLGA),加载无细胞脂肪提取物（Ceffe）。Ceffe已经证明了促进血管生成和细胞增殖的能力，使其成为生物打印精确乳头状软骨移植物的理想生物墨水。我们利用Ceffe/PLGA支架来创建具有精确乳头形状的多孔结构。该支架表现出良好的孔隙率和孔径，确保稳定的形状维护和令人满意的生物力学特性。重要的是,它可以持续释放Seffe.我们的体外实验结果证实了支架良好的生物相容性和促进血管生成的能力,如支持软骨细胞增殖和内皮细胞迁移和管形成所证明的。此外，体内培养8周后，Seffe/PLGA支架接种软骨细胞，再生为具有精确乳头形状的软骨支撑结构。与纯PLGA组相比，Ceffe/PLGA支架显示显著的血管形成，突出塞夫的有益效果。这些发现表明，我们设计的具有乳头形状的Ceffe/PLGA支架代表了精确乳头状软骨再生的有希望的策略。为后续乳头重建奠定基础。
