PDF(Pubmed)
Abstract：
OBJECTIVE: Given the characteristics of Serratia marcescens (S. marcescens), this study aimed at investigating its presence in the hands and contact lens cases of orthokeratology wearers, along with the status of bacterial contamination.
METHODS: The 39 patients received the questionnaires about the background of orthokeratology and hygiene habits. A total of 39 contact lens cases and 39 hand samples from the patients were collected at Show Chwan Memorial Hospital from June to August in 2020 and sent to National Chung Cheng University for DNA extraction and PCR identification.
RESULTS: The results indicated a detection rate of 5.13% for S. marcescens in the contact lens cases and 12.82% in the hand samples. Additionally, 66.67% of contact lens case samples and 30.77% of hand samples found positive for 16s bacterial amplicons. The relationship between hand contamination and the duration of contact lens usage were revealed for both S. marcescens (p=0.021) and 16s bacterial amplicons (p=0.048).
CONCLUSIONS: The results indicated that hand hygiene is more critical than focusing on contact lens hygiene when it comes to preventing S. marcescens infections. Nevertheless, both proper hand and contact lens hygiene practices can reduce the detection of bacterial eye pathogens, especially a common intestinal bacterium.
METHODS: The 39 patients received the questionnaires about the background of orthokeratology and hygiene habits. A total of 39 contact lens cases and 39 hand samples from the patients were collected at Show Chwan Memorial Hospital from June to August in 2020 and sent to National Chung Cheng University for DNA extraction and PCR identification.
RESULTS: The results indicated a detection rate of 5.13% for S. marcescens in the contact lens cases and 12.82% in the hand samples. Additionally, 66.67% of contact lens case samples and 30.77% of hand samples found positive for 16s bacterial amplicons. The relationship between hand contamination and the duration of contact lens usage were revealed for both S. marcescens (p=0.021) and 16s bacterial amplicons (p=0.048).
CONCLUSIONS: The results indicated that hand hygiene is more critical than focusing on contact lens hygiene when it comes to preventing S. marcescens infections. Nevertheless, both proper hand and contact lens hygiene practices can reduce the detection of bacterial eye pathogens, especially a common intestinal bacterium.
摘要：
目的：鉴于粘质沙雷氏菌的特征（S.marcescens),这项研究旨在调查角膜塑形镜佩戴者的手和隐形眼镜中存在的情况，以及细菌污染的状况。
方法：39例患者接受了关于角膜塑形术背景和卫生习惯的问卷调查。2020年6月至8月，在ShowChwan纪念医院收集了39例隐形眼镜病例和39例患者的手部样本，并送往国立中正大学进行DNA提取和PCR鉴定。
结果：结果表明，粘质链球菌在隐形眼镜病例中的检出率为5.13％，在手样本中的检出率为12.82％。此外,66.67％的隐形眼镜病例样本和30.77％的手样本发现16s细菌扩增子呈阳性。对于粘质链球菌（p=0.021）和16s细菌扩增子（p=0.048），揭示了手污染与隐形眼镜使用持续时间之间的关系。
结论：结果表明，在预防粘质链球菌感染方面，手部卫生比注重隐形眼镜卫生更为重要。然而，适当的手部和隐形眼镜卫生习惯可以减少细菌性眼部病原体的检测，尤其是常见的肠道细菌.
方法：39例患者接受了关于角膜塑形术背景和卫生习惯的问卷调查。2020年6月至8月，在ShowChwan纪念医院收集了39例隐形眼镜病例和39例患者的手部样本，并送往国立中正大学进行DNA提取和PCR鉴定。
结果：结果表明，粘质链球菌在隐形眼镜病例中的检出率为5.13％，在手样本中的检出率为12.82％。此外,66.67％的隐形眼镜病例样本和30.77％的手样本发现16s细菌扩增子呈阳性。对于粘质链球菌（p=0.021）和16s细菌扩增子（p=0.048），揭示了手污染与隐形眼镜使用持续时间之间的关系。
结论：结果表明，在预防粘质链球菌感染方面，手部卫生比注重隐形眼镜卫生更为重要。然而，适当的手部和隐形眼镜卫生习惯可以减少细菌性眼部病原体的检测，尤其是常见的肠道细菌.
