Abstract：
The interaction of L-Phe with the membrane components, i.e., lipids and proteins, has been discussed in the current literature due to the interest to understand the effect of single amino acids in relation to the formation of amyloid aggregates. In the present work, it is shown that L-Phe interacts with 9:1 DMPC (1,2-dimyristoyl-sn-glycero-3 phosphocholine)/DPPC (1,2-dipalmitoyl-sn-glycero-3 phosphocholine) mixtures but not in the 1:9 one. An important observation is that the interaction disappears when DPPC is replaced by diether PC (2-di-O-hexadecyl-sn-glycero-3-phosphocholine) a lipid lacking carbonyl groups (CO). This denotes that CO groups may interact specifically with L-Phe in accordance with the appearance of a new peak observed by Infrared spectroscopy (FTIR-ATR). The interaction of L-Phe affects the compressibility pattern of the 9:1 DMPC/DPPC mixture which is congruent with the changes observed by Raman spectra. The specific interaction of L-Phe with CO, propagates to phosphate and choline groups in this particular mixture as analyzed by FTIR-ATR spectroscopy and is absent when DMPC is dopped with diether PC.
摘要：
L-Phe与膜成分的相互作用，即，脂质和蛋白质，由于有兴趣了解单个氨基酸对淀粉样蛋白聚集体形成的影响，因此在当前文献中进行了讨论。在目前的工作中,表明L-Phe与9：1DMPC（1,2-二肉豆蔻酰-sn-甘油-3磷酸胆碱）/DPPC（1,2-二棕榈酰-sn-甘油-3磷酸胆碱）混合物相互作用，但不在1：9之一。一个重要的观察结果是，当DPPC被二醚PC（2-二-O-十六烷基-sn-甘油-3-磷酸胆碱）取代时，相互作用消失了，这是一种缺乏羰基（CO）的脂质。这表示CO基团可以根据通过红外光谱法(FTIR-ATR)观察到的新峰的出现而与L-Phe特异性地相互作用。L-Phe的相互作用影响9：1DMPC/DPPC混合物的可压缩性模式，该模式与拉曼光谱观察到的变化一致。L-Phe与CO的特异性相互作用，如通过FTIR-ATR光谱分析的，在该特定混合物中传播到磷酸盐和胆碱基团，并且当DMPC掺杂二醚PC时不存在。
