Abstract：
The photosynthetic autotrophic production of microalgae is limited by the effective supply of carbon and light energy, and the production efficiency is lower than the theoretical value. Represented by methanol, C1 compounds have been industrially produced by artificial photosynthesis with a solar energy efficiency over 10%, but the complexity of artificial products is weak. Here, based on a construction of chloroplast factory, green microalgae Chlamydomonas reinhardtii CC137c was modified for the bioconversion of formate for biomass production. By screening the optimal combination of chloroplast transport peptides, the cabII-1 cTP1 fusion formate dehydrogenase showed significant enhancement on the conversion of formate with a better performance in the maintenance of light reaction activity. This work provided a new way to obtain bioproducts from solar energy and CO2 with potentially higher-than-nature efficiency by the artificial-natural hybrid photosynthesis.
摘要：
微藻的光合自养生产受限于碳和光能的有效供应,生产效率低于理论值。以甲醇为代表，C1化合物已通过人工光合作用工业生产，太阳能效率超过10％，但是人造产品的复杂性很弱。这里,基于叶绿体工厂的建设，对绿色微藻莱茵衣藻CC137c进行了修饰，以将甲酸生物转化用于生物质生产。通过筛选叶绿体转运肽的优化组合,cabII-1cTP1融合甲酸脱氢酶对甲酸的转化率显着增强，在维持光反应活性方面表现更好。这项工作提供了一种通过人工-自然混合光合作用从太阳能和二氧化碳中获得生物产品的新途径，其效率可能高于自然。
