Abstract：
BACKGROUND: Vascular catheter-related infections and thrombosis are common and may lead to serious complications after catheterization. Reducing the incidence of such infections has become a significant challenge.
OBJECTIVE: This study aims to develop a super hydrophobic nanocomposite drug-loaded vascular catheter that can effectively resist bacterial infections and blood coagulation.
METHODS: In this study, a SiO2 nanocoated PTFE (Polytetrafluoroethylene) catheter (PTFE-SiO2) was prepared and further optimized to prepare a SiO2 nanocoated PTFE catheter loaded with imipenem/cilastatin sodium (PTFE-IC@dMSNs). The catheters were characterized for performance, cell compatibility, anticoagulant performance, in vitro and in vivo antibacterial effect and biological safety.
RESULTS: PTFE-IC@dMSNs catheter has efficient drug loading performance and drug release rate and has good cell compatibility and anticoagulant effect in vitro. Compared with the PTFE-SiO2 catheter, the inhibition ring of the PTFE-IC@dMSNs catheter against Escherichia coli increased from 3.98 mm2 to 4.56 mm2, and the antibacterial rate increased from about 50.8 % to 56.9 %, with a significant difference (p < 0.05). The antibacterial zone against Staphylococcus aureus increased from 8.63 mm2 to 11.74 mm2, and the antibacterial rate increased from approximately 83.5 % to 89.3 %, showing a significant difference (p < 0.05). PTFE-IC@dMSNs catheter also has good biocompatibility in vivo. Furthermore, the PTFE-IC@dMSNs catheter can reduce the adhesion of blood cells and have excellent anticoagulant properties, and even maintain these properties even with the addition of imipenem/cilastatin sodium.
CONCLUSIONS: Compared with PTFE, PTFE-SiO2 and PTFE-IC@dMSNs catheters have good characterization performance, cell compatibility, and anticoagulant properties. PTFE SiO2 and PTFE-IC@dMSNs catheters have good antibacterial performance and tissue safety against E. coli and S. aureus. Relatively, PTFE-SiO2 and PTFE-IC@dMSNs catheter has better antibacterial properties and histocompatibility and has potential application prospects in anti-bacterial catheter development and anticoagulation.
OBJECTIVE: This study aims to develop a super hydrophobic nanocomposite drug-loaded vascular catheter that can effectively resist bacterial infections and blood coagulation.
METHODS: In this study, a SiO2 nanocoated PTFE (Polytetrafluoroethylene) catheter (PTFE-SiO2) was prepared and further optimized to prepare a SiO2 nanocoated PTFE catheter loaded with imipenem/cilastatin sodium (PTFE-IC@dMSNs). The catheters were characterized for performance, cell compatibility, anticoagulant performance, in vitro and in vivo antibacterial effect and biological safety.
RESULTS: PTFE-IC@dMSNs catheter has efficient drug loading performance and drug release rate and has good cell compatibility and anticoagulant effect in vitro. Compared with the PTFE-SiO2 catheter, the inhibition ring of the PTFE-IC@dMSNs catheter against Escherichia coli increased from 3.98 mm2 to 4.56 mm2, and the antibacterial rate increased from about 50.8 % to 56.9 %, with a significant difference (p < 0.05). The antibacterial zone against Staphylococcus aureus increased from 8.63 mm2 to 11.74 mm2, and the antibacterial rate increased from approximately 83.5 % to 89.3 %, showing a significant difference (p < 0.05). PTFE-IC@dMSNs catheter also has good biocompatibility in vivo. Furthermore, the PTFE-IC@dMSNs catheter can reduce the adhesion of blood cells and have excellent anticoagulant properties, and even maintain these properties even with the addition of imipenem/cilastatin sodium.
CONCLUSIONS: Compared with PTFE, PTFE-SiO2 and PTFE-IC@dMSNs catheters have good characterization performance, cell compatibility, and anticoagulant properties. PTFE SiO2 and PTFE-IC@dMSNs catheters have good antibacterial performance and tissue safety against E. coli and S. aureus. Relatively, PTFE-SiO2 and PTFE-IC@dMSNs catheter has better antibacterial properties and histocompatibility and has potential application prospects in anti-bacterial catheter development and anticoagulation.
摘要：
背景：血管导管相关感染和血栓形成是常见的，并可能导致导管插入后的严重并发症。降低此类感染的发生率已成为重大挑战。
目的：本研究旨在开发一种超疏水纳米复合材料载药血管导管，该导管可有效抵抗细菌感染和血液凝固。
方法：在本研究中，制备SiO2纳米涂覆的PTFE(聚四氟乙烯)导管(PTFE-SiO2)并进一步优化以制备负载有亚胺培南/西司他汀钠的SiO2纳米涂覆的PTFE导管(PTFE-IC@dMSNs)。导管的性能进行了表征，细胞相容性，抗凝血性能,体内外抗菌作用和生物安全性。
结果：PTFE-IC@dMSNs导管具有高效的载药性能和药物释放速率，在体外具有良好的细胞相容性和抗凝血作用。与PTFE-SiO2导管相比，PTFE-IC@dMSNs导管对大肠杆菌的抑制环从3.98mm2增加到4.56mm2，抗菌率从约50.8%增加到56.9%，具有显著性差异(p<0.05)。对金黄色葡萄球菌的抗菌区从8.63mm2增加到11.74mm2,抗菌率从约83.5%增加到89.3%,显着差异(p<0.05)。PTFE-IC@dMSNs导管在体内也具有良好的生物相容性。此外，PTFE-IC@dMSNs导管可以减少血细胞的粘附，具有优异的抗凝血性能，甚至在加入亚胺培南/西司他丁钠的情况下也能保持这些性能。
结论：与PTFE相比，PTFE-SiO2和PTFE-IC@dMSNs导管具有良好的表征性能，细胞相容性，和抗凝血性能。PTFESiO2和PTFE-IC@dMSNs导管对大肠杆菌和金黄色葡萄球菌具有良好的抗菌性能和组织安全性。相对而言,PTFE-SiO2和PTFE-IC@dMSNs导管具有较好的抗菌性能和组织相容性,在抗菌导管研制和抗凝方面具有潜在的应用前景。
目的：本研究旨在开发一种超疏水纳米复合材料载药血管导管，该导管可有效抵抗细菌感染和血液凝固。
方法：在本研究中，制备SiO2纳米涂覆的PTFE(聚四氟乙烯)导管(PTFE-SiO2)并进一步优化以制备负载有亚胺培南/西司他汀钠的SiO2纳米涂覆的PTFE导管(PTFE-IC@dMSNs)。导管的性能进行了表征，细胞相容性，抗凝血性能,体内外抗菌作用和生物安全性。
结果：PTFE-IC@dMSNs导管具有高效的载药性能和药物释放速率，在体外具有良好的细胞相容性和抗凝血作用。与PTFE-SiO2导管相比，PTFE-IC@dMSNs导管对大肠杆菌的抑制环从3.98mm2增加到4.56mm2，抗菌率从约50.8%增加到56.9%，具有显著性差异(p<0.05)。对金黄色葡萄球菌的抗菌区从8.63mm2增加到11.74mm2,抗菌率从约83.5%增加到89.3%,显着差异(p<0.05)。PTFE-IC@dMSNs导管在体内也具有良好的生物相容性。此外，PTFE-IC@dMSNs导管可以减少血细胞的粘附，具有优异的抗凝血性能，甚至在加入亚胺培南/西司他丁钠的情况下也能保持这些性能。
结论：与PTFE相比，PTFE-SiO2和PTFE-IC@dMSNs导管具有良好的表征性能，细胞相容性，和抗凝血性能。PTFESiO2和PTFE-IC@dMSNs导管对大肠杆菌和金黄色葡萄球菌具有良好的抗菌性能和组织安全性。相对而言,PTFE-SiO2和PTFE-IC@dMSNs导管具有较好的抗菌性能和组织相容性,在抗菌导管研制和抗凝方面具有潜在的应用前景。
