Abstract：
BACKGROUND: Rhubarb is widely distributed and cultivated worldwide, and its leaves presented antioxidant activity and could be used as food additive. However, the chemical ingredients, and protective effect of Rheum officinale leaf juice (JROL) on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) are still unclear.
OBJECTIVE: This paper sought to the characterization and functional properties of JROL, and explore the underlying mechanism on UC mice.
METHODS: UPLC-ESI-Q-TOF/MS and other analytical instruments were employed to determine the chemical ingredients of JROL. After inducing UC model using 3% DSS, multiple biological methods were used to evaluate its protective effect and the potential mechanism.
RESULTS: JROL is rich in proximate compositions and minerals and has high nutritional value, and contains reducing sugars, polysaccharides and pectin. Fifteen compounds were identified using UPLC-ESI-Q-TOF/MS. Among them, rutin has the highest content (2.22 %) in UPLC analysis. JROL presented protective effect on DSS-induced UC, and alleviated morphological alterations and ultra-structural feature of tissue, and the polysaccharides and flavonoids may contribute to its protective effect. JROL inhibited NF-κB/NLRP3 signaling pathway to alleviate inflammatory response, oxidative stress and intestinal injury by decreasing the expression of p-p65, p-IκBα, NLRP3, ASC, etc.. Moreover, it up-regulated the expression of tight junction proteins, and re-balanced the disturbance of gut microbiota to regulate the inflammatory response. Finally, a correlation among the inflammatory response, NF-κB/NLRP3 pathway and gut microbiota was established. Moreover, JROL presented the safety in the acute toxicity test.
CONCLUSIONS: JROL could be used as a potential new source for treating UC.
OBJECTIVE: This paper sought to the characterization and functional properties of JROL, and explore the underlying mechanism on UC mice.
METHODS: UPLC-ESI-Q-TOF/MS and other analytical instruments were employed to determine the chemical ingredients of JROL. After inducing UC model using 3% DSS, multiple biological methods were used to evaluate its protective effect and the potential mechanism.
RESULTS: JROL is rich in proximate compositions and minerals and has high nutritional value, and contains reducing sugars, polysaccharides and pectin. Fifteen compounds were identified using UPLC-ESI-Q-TOF/MS. Among them, rutin has the highest content (2.22 %) in UPLC analysis. JROL presented protective effect on DSS-induced UC, and alleviated morphological alterations and ultra-structural feature of tissue, and the polysaccharides and flavonoids may contribute to its protective effect. JROL inhibited NF-κB/NLRP3 signaling pathway to alleviate inflammatory response, oxidative stress and intestinal injury by decreasing the expression of p-p65, p-IκBα, NLRP3, ASC, etc.. Moreover, it up-regulated the expression of tight junction proteins, and re-balanced the disturbance of gut microbiota to regulate the inflammatory response. Finally, a correlation among the inflammatory response, NF-κB/NLRP3 pathway and gut microbiota was established. Moreover, JROL presented the safety in the acute toxicity test.
CONCLUSIONS: JROL could be used as a potential new source for treating UC.
摘要：
背景：大黄在世界范围内广泛分布和栽培，其叶片具有抗氧化活性，可用作食品添加剂。然而,化学成分，大黄叶汁（JROL）对葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）的保护作用尚不清楚。
目的：本文对JROL,并探讨其对UC小鼠的作用机制。
方法：采用UPLC-ESI-Q-TOF/MS等分析仪器测定JROL的化学成分。使用3%DSS诱导UC模型后，采用多种生物学方法评价其保护作用和潜在机制。
结果：JROL富含近似成分和矿物质，具有很高的营养价值，含有还原糖，多糖和果胶。使用UPLC-ESI-Q-TOF/MS鉴定了15种化合物。其中,芦丁在UPLC分析中含量最高(2.22%)。JROL对DSS诱导的UC有保护作用，减轻组织的形态改变和超结构特征，多糖和类黄酮可能有助于其保护作用。JROL抑制NF-κB/NLRP3信号通路减轻炎症反应,氧化应激和肠损伤通过降低p-p65,p-IκBα的表达,NLRP3,ASC,等。.此外，它上调了紧密连接蛋白的表达，并重新平衡肠道菌群的紊乱以调节炎症反应。最后,炎症反应之间的相关性，建立了NF-κB/NLRP3通路和肠道菌群。此外，JROL在急性毒性试验中提出了安全性。
结论：JROL可作为治疗UC的潜在新来源。
目的：本文对JROL,并探讨其对UC小鼠的作用机制。
方法：采用UPLC-ESI-Q-TOF/MS等分析仪器测定JROL的化学成分。使用3%DSS诱导UC模型后，采用多种生物学方法评价其保护作用和潜在机制。
结果：JROL富含近似成分和矿物质，具有很高的营养价值，含有还原糖，多糖和果胶。使用UPLC-ESI-Q-TOF/MS鉴定了15种化合物。其中,芦丁在UPLC分析中含量最高(2.22%)。JROL对DSS诱导的UC有保护作用，减轻组织的形态改变和超结构特征，多糖和类黄酮可能有助于其保护作用。JROL抑制NF-κB/NLRP3信号通路减轻炎症反应,氧化应激和肠损伤通过降低p-p65,p-IκBα的表达,NLRP3,ASC,等。.此外，它上调了紧密连接蛋白的表达，并重新平衡肠道菌群的紊乱以调节炎症反应。最后,炎症反应之间的相关性，建立了NF-κB/NLRP3通路和肠道菌群。此外，JROL在急性毒性试验中提出了安全性。
结论：JROL可作为治疗UC的潜在新来源。
