METHODS: Meningococcal isolates were collected through national, laboratory-based invasive meningococcal disease (IMD) surveillance. Phenotypic antimicrobial susceptibility testing and whole-genome sequencing were performed, and the mechanism of reduced penicillin susceptibility was assessed in silico.
RESULTS: Of 585 IMD cases reported during the study period, culture and PCR-based capsular group was determined for 477/585 (82%); and 241/477 (51%) were sequenced. Predominant serogroups included NmB (210/477; 44%), NmW (116/477; 24%), NmY (96/477; 20%) and NmC (48/477; 10%). Predominant clonal complexes (CC) were CC41/44 in NmB (27/113; 24%), CC11 in NmW (46/56; 82%), CC167 in NmY (23/44; 53%), and CC865 in NmC (9/24; 38%). Peni was detected in 16% (42/262) of isolates, and was due to the presence of a penA mosaic, with the majority harboring penA7, penA9 or penA14.
CONCLUSIONS: IMD lineages circulating in South Africa were consistent with those circulating prior to 2008, however peni was higher than previously reported, and occurred in a variety of lineages.
方法:通过国家收集脑膜炎球菌分离株,基于实验室的侵袭性脑膜炎球菌病(IMD)监测。进行了表型抗菌素药敏试验和全基因组测序,并对青霉素敏感性降低的机制进行了计算机评估。
结果:在研究期间报告的585例IMD病例中,培养和基于PCR的荚膜组确定为477/585(82%);并对241/477(51%)进行了测序。主要血清群包括NmB(210/477;44%),NmW(116/477;24%),NmY(96/477;20%)和NmC(48/477;10%)。主要克隆复合物(CC)是NmB中的CC41/44(27/113;24%),CC11单位:NmW(46/56;82%),CC167在NmY(23/44;53%),NmC中的CC865(9/24;38%)。在16%(42/262)的分离物中检测到Peni,是由于pena马赛克的存在,其中大多数拥有penA7,penA9或penA14。
结论:在南非传播的IMD谱系与2008年之前的谱系一致,但是peni比以前报道的要高,发生在各种谱系中。