Abstract：
Borrelia miyamotoi disease is an emerging tick-borne human illness in the United States caused by Borrelia miyamotoi (Spirochaetales: Spirochaetaceae) bacterium. With Pennsylvania reporting thousands of tick-borne disease cases annually, determining the minimum infection rate (MIR) of B. miyamotoi in Ixodes scapularis (Say, Acari: Ixodidae) adults within Pennsylvania is of utmost importance. Active surveillance was performed from October 2019 to April 2020 to collect a minimum of 50 I. scapularis ticks from every county within Pennsylvania and then screened for B. miyamotoi via qPCR. Ticks were collected from all 67 counties with the majority of those being adult I. scapularis. Additional ticks collected were Dermacentor albipictus (Packard, Acari: Ixodidae), Haemaphysalis longicornis (Neumann, Acari: Ixodidae), and immature I. scapularis. Adult I. scapularis were pooled and tested for B. miyamotoi. MIR for positive B. miyamotoi pools and density of infected adult I. scapularis varied by county, with positive pools from 38 Pennsylvania counties. This is the first statewide evaluation of B. miyamotoi in Pennsylvania in questing adult I. scapularis. These prevalence and distribution data will aid health care practitioners within the state of Pennsylvania and the northeast United States to understand potential risk and bring awareness to the lesser known human Borrelia illness, Borrelia miyamotoi disease.
摘要：
miyamotoi病是在美国由miyamotoi（Spirochaetales：Spirochaetaceae）细菌引起的一种新兴的蜱传人类疾病。宾夕法尼亚州每年报告数千起蜱传疾病病例，确定肩胛骨Ixodes中miyamotoiB.miyamotoi的最低感染率（MIR）（说，Acari：Ixodidae）宾夕法尼亚州的成年人至关重要。从2019年10月至2020年4月进行了主动监测，从宾夕法尼亚州的每个县收集至少50个肩胛骨蜱，然后通过qPCR筛选B.miyamotoi。蜱虫是从所有67个县收集的，其中大多数是成年的肩胛骨。收集到的其他蜱虫是阿比皮克人(Packard，Acari：Ixodidae），长尾血齿（Neumann，Acari：Ixodidae），和未成熟的肩胛骨。将成年肩胛骨肌群合并并测试B.miyamotoi。MIR阳性B.miyamotoi池和受感染的成年I.肩胛骨的密度因县而异，来自宾夕法尼亚州38个县的阳性池。这是宾夕法尼亚州对B.miyamotoi进行的首次全州范围内的评估，以寻求成年的肩cap肌。这些患病率和分布数据将帮助宾夕法尼亚州和美国东北部的医疗保健从业人员了解潜在风险，并提高对鲜为人知的人类疏螺旋体病的认识。密螺旋体病。
