Abstract：
Silver has long been recognized for its potent antimicrobial properties, but achieving a slow and longer-term delivery of silver ions presents significant challenges. Previous efforts to control silver ion dosages have struggled to sustain release for extended periods in biomimetic environments, especially in the presence of complex proteins. This challenge is underscored by the absence of technology for sustaining antimicrobial activity, especially in the context of orthopedic implants where long-term efficacy, extending beyond 7 days, is essential. In this study, the tunable, slow, and longer-term release of silver ions from the two-dimensional (2D) nanocapillaries of graphene oxide (GO) laminates incorporated with silver ions (Ag-GO) for antimicrobial applications are successfully demonstrated. To closely mimic a physiologically relevant serum-based environment, a novel in vitro study model using 100% fetal bovine serum (FBS) is introduced as the test medium for microbiology, biocompatibility, and bioactivity studies. To emulate fluid circulation in a physiological environment, the in vitro studies are challenged with serum exchange protocols on different days. The findings show that the Ag-GO coating can sustainably release silver ions at a minimum dosage of 10 µg cm-2 day-1, providing an effective and sustained antimicrobial barrier for over ten days.
摘要：
长期以来，银因其有效的抗菌性能而被认可，但是实现银离子的缓慢和长期递送提出了重大挑战。以前控制银离子剂量的努力一直难以在仿生环境中长时间持续释放。特别是在复杂蛋白质的存在下。缺乏维持抗菌活性的技术突出了这一挑战，特别是在骨科植入物的长期疗效，超过7天，是必不可少的。在这项研究中,可调的，慢,成功地证明了银离子从与银离子（Ag-GO）结合的氧化石墨烯（GO）层压板的二维（2D）纳米毛细管中的长期释放，用于抗菌应用。为了密切模仿生理相关的血清环境，采用100%胎牛血清(FBS)作为微生物学试验培养基的新型体外研究模型,生物相容性，和生物活性研究。为了模拟生理环境中的液体循环，体外研究在不同的日子受到血清交换方案的挑战。研究结果表明，Ag-GO涂层可以以10µgcm-2day-1的最小剂量持续释放银离子，提供了超过十天的有效和持续的抗菌屏障。
