Abstract：
Calcium supplementation has been shown to be efficacious in mitigating the progression of senile osteoporosis (SOP) and reducing the incidence of osteoporotic fractures resulting from prolonged calcium shortage. In this study, Grifola frondosa (GF) peptides-calcium chelate were synthesized through the interaction between peptide from GF and CaCl2. The chelation reaction was shown to involve the participation of the amino and carboxyl groups in the peptide, as revealed by scanning electron microscope, Fourier-transform infrared, and ultraviolet spectrophotometry. Furthermore, a mouse model of (SOP) induced by d-galactose was established (SCXK-2018-0004). Results demonstrated that low dosage of low-molecular weight GF peptides-calcium chelates (LLgps-Ca) could significantly improve serum index and pathological features of bone tissue and reduce bone injury. Further research suggested that LLgps-Ca could ameliorate SOP by modulating the disrupted metabolic pathway, which includes focal adhesion, extracellular matrix receptor interaction, and PI3K-Akt signaling pathway. Using Western blot, the differentially expressed proteins were further confirmed. Thus, calciumchelating peptides from GF could serve as functional calcium agents to alleviate SOP.
摘要：
钙补充剂已被证明可有效缓解老年性骨质疏松症（SOP）的进展，并减少因长期钙短缺而导致的骨质疏松性骨折的发生率。在这项研究中,通过来自GF和CaCl2的肽之间的相互作用，合成了灰树花（GF）肽-钙螯合物。显示螯合反应涉及肽中氨基和羧基的参与，扫描电子显微镜显示，傅里叶变换红外,和紫外分光光度法。此外，建立d-半乳糖诱导的SOP小鼠模型(SCXK-2018-0004)。结果表明，低剂量低分子量GF肽-钙螯合物（LLgps-Ca）可明显改善血清指标和骨组织病理特征，减少骨损伤。进一步的研究表明，LLgps-Ca可以通过调节被破坏的代谢途径来改善SOP，其中包括局灶性粘连，细胞外基质受体相互作用，和PI3K-Akt信号通路。使用蛋白质印迹，进一步证实了差异表达的蛋白质。因此，来自GF的钙螯合肽可以作为功能性钙剂来缓解SOP。
