Abstract：
Fowl Pox Viruses (FPV) infect chickens and turkeys giving rise to pock lesions on various body parts like combs, wattles, legs, shanks, eyes, mouth etc. The birds, affected with FPV, also show anemia and ruffled appearance which are clinical symptoms of Reticuloendotheliosis. Interestingly, the field strains of FPV are integrated with the provirus of Reticuloendotheliosis Virus (REV). Due to this integration, the infected birds, upon replication of FPV, give rise to free REV virions, causing severe immunosuppression and anemia. Pox scabs, collected from the infected birds, not only show positive PCR results upon performing FPV-specific 4b core protein gene PCR but also show positive results for the PCR of REV-specific env gene and FPV-REV 5\'LTR junction. Homogenized suspension of the pock lesions, upon inoculating to the Chorio-allantoic Membrane (CAM) of 10 days old specific pathogen-free embryonated chicken eggs, produces characteristic pock lesions in serial passages. But the lesions also harbor REV mRNA or free virion, which can be identified by performing REV-specific env gene PCR using REV RNA from FPV-infected CAMs. The study suggests successful replication and availability of REV mRNA and free virion alongside the FPV virus, although the CAM is an ill-suited medium for any retroviral (like REV) growth and replication.
摘要：
鸡痘病毒（FPV）感染鸡和火鸡，在各个身体部位如梳子上引起麻子损伤，wattles,腿,Shanks,眼睛,嘴等。鸟，受FPV影响，还显示贫血和起皱的外观，这是网状内皮增生的临床症状。有趣的是,FPV的田间毒株与网状内皮组织增生症病毒（REV）的前病毒整合。由于这种整合，受感染的鸟类,在FPV复制后，产生自由的REV病毒体，导致严重的免疫抑制和贫血。痘痘痂,从受感染的鸟类中收集，不仅在进行FPV特异性4b核心蛋白基因PCR时显示阳性PCR结果，而且在REV特异性env基因和FPV-REV5'LTR连接的PCR中也显示阳性结果。麻点病变的均质悬浮液，接种10天大的无特定病原体的含胚鸡蛋的绒毛尿囊膜（CAM）后，在连续通道中产生特征性的麻点病变。但是病变也带有REVmRNA或游离病毒体，whichcanbeidentifiedbyperformingREV-specificenvgenePCRusingREVRNAfromFPV-infectedCAMs.ThestudysuggestedsuccessfulreplicationandavailabilityofREVmRNAandfreevirionalongtheFPVvirus,尽管CAM是不适合任何逆转录病毒（如REV）生长和复制的培养基。
