Abstract：
As an agricultural pest, the fall armyworm (FAW), Spodoptera frugiperda, poses a severe threat to agriculture in China. Chlorantraniliprole has been widely used to control this pest. In our previous studies, we discovered that LD10, LD20, and LD30 chlorantraniliprole promoted encapsulation in the 4th instar larvae of the FAW, with LD30 chlorantraniliprole having the most significant effect. To further investigate the molecular mechanism underlying the sublethal effects of chlorantraniliprole on encapsulation in the FAW, this study conducted the effects of encapsulation in 4th instar larvae of the FAW exposed to LD30 chlorantraniliprole. Then, we analyzed the transcriptome of the FAW hemolymph treated with LD30 chlorantraniliprole and identified genes related to encapsulation using RNAi. Our results showed that the encapsulation in the FAW was enhanced at 6, 12, 18, 24, and 48 h after exposure to LD30 chlorantraniliprole. Additionally, LD30 chlorantraniliprole significantly affected the expression of certain immune-related genes, with the heat shock protein 70 family gene SfHSP68.1 showing the most significant upregulation. Subsequent interference with SfHSP68.1 resulted in a significant inhibition of encapsulation in FAW. These findings suggested that LD30 chlorantraniliprole can promote encapsulation in the FAW by upregulating SfHSP68.1 expression. This study provides valuable insights into the sublethal effects of chlorantraniliprole on encapsulation in the FAW and the interaction between encapsulation and heat shock proteins (HSPs).
摘要：
作为一种农业害虫，秋季粘虫(一汽)，节食夜蛾,对中国农业构成严重威胁。Chloantraniliprole已被广泛用于控制这种害虫。在我们之前的研究中,我们发现LD10，LD20和LD30的chloantraniliprole促进了一汽4龄幼虫的包封，以LD30的氯丁虫效果最显著。为了进一步研究氯苯三虫亚致死作用的分子机制，这项研究对暴露于LD30的FAW第四龄幼虫进行了包封作用。然后，我们分析了用LD30chloantraniliprole处理的FAW血淋巴的转录组，并使用RNAi鉴定了与封装相关的基因。我们的结果表明，在暴露于LD30chloantraniliprole后6、12、18、24和48小时，FAW中的封装得到了增强。此外,LD30对某些免疫相关基因的表达有显著影响,热休克蛋白70家族基因SfHSP68.1表现出最显著的上调。随后对SfHSP68.1的干扰导致FAW中包封的显著抑制。这些发现表明，LD30chloantraniliprole可以通过上调SfHSP68.1表达来促进FAW中的包封。这项研究提供了有价值的见解，以了解氯连虫对FAW中封装的亚致死作用以及封装与热休克蛋白（HSP）之间的相互作用。
