Abstract：
The phenomenon of sex chromosome loss from hematopoietic cells is an emerging indicator of biological aging. While many methods to detect this loss have been developed, enhancing the field, these existing methods often suffer from being labor-intensive, expensive, and not sufficiently sensitive. To bridge this gap, a novel and more efficient technique is developed, named the SinChro assay. This method employs multiplexed single-cell droplet PCR, designed to detect cells with sex chromosome loss at single-cell resolution. Through the SinChro assay, the age-dependent increase in Y chromosome loss in male blood is successfully mapped. The age-dependent loss of the X chromosome in female blood is also identified, a finding that has been challenging with existing methods. The advent of the SinChro assay marks a significant breakthrough in the study of age-related sex mosaicism. Its utility extends beyond blood analysis, applicable to a variety of tissues, and it holds the potential to deepen the understanding of biological aging and related diseases.
摘要：
造血细胞的性染色体丢失现象是生物衰老的新兴指标。虽然已经开发了许多检测这种损失的方法，加强领域，这些现有的方法往往是劳动密集型的，贵,不够敏感。为了弥合这个差距，开发了一种新颖且更有效的技术，命名为SinChro试验。该方法采用多重单细胞液滴PCR，旨在以单细胞分辨率检测性染色体丢失的细胞。通过SinChro检测，成功定位了男性血液中Y染色体缺失的年龄依赖性增加。还确定了女性血液中X染色体的年龄依赖性丢失，这一发现对现有方法一直具有挑战性。SinChro测定法的出现标志着与年龄相关的性别镶嵌性研究的重大突破。它的效用超出了血液分析，适用于各种组织，它有可能加深对生物衰老和相关疾病的理解。
