Abstract：
Some forage legumes synthesize phytoestrogens. We conducted a glasshouse study to investigate how water stress (drought and waterlogging) influences phytoestrogen accumulation in red clover and kura clover. Compared to the red clover control, the 20 day drought resulted in an over 100% increase in the phytoestrogens formononetin and biochanin A, which together accounted for 91-96% of the total phytoestrogens measured. Waterlogging resulted in elevated concentrations of daidzein, genistein, and prunetin but not formononetin or biochanin A. Concentrations of phytoestrogens in kura clover were low or undetectable, regardless of water stress treatment. Leaf water potential was the most explanatory single-predictor of the variation in concentrations of formononetin, biochanin A, and total phytoestrogens in red clover. These results suggest that drought-stressed red clover may have higher potential to lead to estrogenic effects in ruminant livestock and that kura clover is a promising alternative low- or no-phytoestrogen perennial forage legume.
摘要：
一些牧草豆科植物合成植物雌激素。我们进行了一项温室研究，以研究水分胁迫（干旱和内涝）如何影响红三叶草和库拉三叶草中植物雌激素的积累。与红三叶草对照相比，20天的干旱导致植物雌激素Formononetin和BiochaninA增加超过100%，它们总共占测量的总植物雌激素的91-96%。淹水导致大豆苷元浓度升高，Genistein,和Prunetin，而不是formononetin或biochaninA.苦拉三叶草中植物雌激素的浓度很低或无法检测到，不管水的压力处理。叶水势是Formonoetin浓度变化的最解释性的单一预测因子，biochaninA,和红三叶草中的总植物雌激素。这些结果表明，干旱胁迫的红三叶草可能有更高的潜力导致反刍动物牲畜的雌激素效应，而库拉三叶草是一种有希望的替代低或无植物雌激素的多年生饲草豆科植物。
