PDF(Pubmed)
Abstract：
Stem cell-based therapies exhibit considerable promise in the treatment of diabetes and its complications. Extensive research has been dedicated to elucidate the characteristics and potential applications of adipose-derived stromal/stem cells (ASCs). Three-dimensional (3D) culture, characterized by rapid advancements, holds promise for efficacious treatment of diabetes and its complications. Notably, 3D cultured ASCs manifest enhanced cellular properties and functions compared to traditional monolayer-culture. In this review, the factors influencing the biological functions of ASCs during culture are summarized. Additionally, the effects of 3D cultured techniques on cellular properties compared to two-dimensional culture is described. Furthermore, the therapeutic potential of 3D cultured ASCs in diabetes and its complications are discussed to provide insights for future research.
摘要：
基于干细胞的疗法在糖尿病及其并发症的治疗中显示出相当大的前景。广泛的研究致力于阐明脂肪来源的基质/干细胞（ASC）的特征和潜在应用。三维（3D）文化，以快速发展为特征，有望有效治疗糖尿病及其并发症。值得注意的是,与传统的单层培养相比，3D培养的ASC表现出增强的细胞特性和功能。在这次审查中,总结了影响ASCs培养过程中生物学功能的因素。此外,描述了与二维培养相比，3D培养技术对细胞特性的影响。此外，讨论了3D培养的ASCs在糖尿病及其并发症中的治疗潜力，为未来的研究提供了见解。
