PDF(Pubmed)
Abstract：
The enteric virome, comprising a complex community of viruses inhabiting the gastrointestinal tract, plays a significant role in health and disease dynamics. In this study, the fecal sample of a wild snow leopard was subjected to viral metagenomic analysis using a double barcode Illumina MiSeq platform. The resulting reads were de novo assembled into contigs with SOAPdenovo2 version r240. Additional bioinformatic analysis of the assembled genome and genome annotation was done using the Geneious prime software (version 2022.0.2). Following viral metagenomic analysis and bioinformatic analysis, a total of 7 viral families and a novel specie of bocaparvovirus tentatively named Panthera uncia bocaparvovirus (PuBOV) with GenBank accession number OQ627713 were identified. The complete genome of PuBOV was predicted to contain 3 open reading frames (ORFs), contains 5433 nucleotides and has a G + C content of 47.40 %. BLASTx analysis and pairwise sequence comparison indicated the novel virus genome was a new species in the genus Bocaparvovirus based on the species demarcation criteria of the International Committee on the Taxonomy of Viruses. This study provides valuable insights into the diversity and composition of the enteric virome in wild endangered snow leopards. The identification and characterization of viruses in wildlife is crucial for developing effective strategies to manage and mitigate potential zoonotic and other viral disease threats to human and animal health.
摘要：
肠道病毒,由居住在胃肠道的复杂病毒群落组成，在健康和疾病动态中起着重要作用。在这项研究中,使用双条形码IlluminaMiSeq平台对野生雪豹的粪便样品进行病毒宏基因组分析.用SOAPdenovo2版本r240将所得读段从头组装成重叠群。使用Geneiousprime软件(版本2022.0.2)对组装的基因组和基因组注释进行额外的生物信息学分析。在病毒宏基因组分析和生物信息学分析之后，共鉴定出7个病毒家族和一种新的博卡病毒，暂定名为Pantherauncia博卡病毒（PuBOV），GenBank登录号为OQ627713。预测PuBOV的完整基因组包含3个开放阅读框（ORF），含有5433个核苷酸并且具有47.40%的G+C含量。BLASTx分析和成对序列比较表明，根据国际病毒分类学委员会的物种划分标准，新病毒基因组是Bocapavovirus属的新物种。这项研究为野生濒危雪豹肠道病毒的多样性和组成提供了有价值的见解。野生动物中病毒的识别和表征对于制定有效的策略来管理和减轻潜在的人畜共患和其他病毒性疾病对人类和动物健康的威胁至关重要。
