PDF(Pubmed)
Abstract：
UNASSIGNED: There is an increasing focus on the prevention of secondary injuries following traumatic spinal cord injury (TSCI), especially through improvement of spinal cord perfusion and immunological modulation. Such therapeutic strategies require translational and controlled animal models of disease progression of the acute phases of human TSCI.
UNASSIGNED: Is it possible to establish a 72-h sedated porcine model of incomplete thoracic TSCI, enabling controlled use of continuous, invasive, and non-invasive modalities during the entire sub-acute phase of TSCI?
UNASSIGNED: A sham-controlled trial was conducted to establish the model, and 10 animals were assigned to either sham or TSCI. All animals underwent a laminectomy, and animals in the TSCI group were subjected to a weight-drop injury. Animals were then kept sedated for 72 h. The amount of injury was assessed by ex-vivo measures MRI-based fiber tractography, histology and immunohistochemistry.
UNASSIGNED: In all animals, we were successful in maintaining sedation for 72 h without comprising vital physiological parameters. The MRI-based fiber tractography showed that all TSCI animals revealed a break in the integrity of spinal neurons, whereas histology demonstrated no transversal sections of the spine with complete injury. Notably, some animals displayed signs of secondary ischemic tissue in the cranial and caudal sections.
UNASSIGNED: This study succeeded in producing a porcine model of incomplete TSCI, which was physiologically stable up to 72 h. We believe that this TSCI model will constitute a potential translational model to study the pathophysiology secondary to TSCI in humans.
UNASSIGNED: Is it possible to establish a 72-h sedated porcine model of incomplete thoracic TSCI, enabling controlled use of continuous, invasive, and non-invasive modalities during the entire sub-acute phase of TSCI?
UNASSIGNED: A sham-controlled trial was conducted to establish the model, and 10 animals were assigned to either sham or TSCI. All animals underwent a laminectomy, and animals in the TSCI group were subjected to a weight-drop injury. Animals were then kept sedated for 72 h. The amount of injury was assessed by ex-vivo measures MRI-based fiber tractography, histology and immunohistochemistry.
UNASSIGNED: In all animals, we were successful in maintaining sedation for 72 h without comprising vital physiological parameters. The MRI-based fiber tractography showed that all TSCI animals revealed a break in the integrity of spinal neurons, whereas histology demonstrated no transversal sections of the spine with complete injury. Notably, some animals displayed signs of secondary ischemic tissue in the cranial and caudal sections.
UNASSIGNED: This study succeeded in producing a porcine model of incomplete TSCI, which was physiologically stable up to 72 h. We believe that this TSCI model will constitute a potential translational model to study the pathophysiology secondary to TSCI in humans.
摘要：
■对创伤性脊髓损伤（TSCI）后继发性损伤的预防越来越重视，特别是通过改善脊髓灌注和免疫调节。这种治疗策略需要人TSCI急性期疾病进展的翻译和受控动物模型。
■是否有可能建立72小时的不完全胸部TSCI镇静猪模型，从而能够连续控制使用，侵入性,在TSCI的整个亚急性期进行非侵入性治疗？
■进行了一项假对照试验以建立模型，和10只动物被分配到假手术或TSCI。所有动物都接受了椎板切除术，TSCI组的动物遭受重量下降损伤。然后将动物镇静72小时。通过基于MRI的离体测量纤维束成像评估损伤量。组织学和免疫组织化学。
■在所有动物中，我们成功地维持了72小时的镇静，而不包括重要的生理参数。基于MRI的纤维束成像显示，所有TSCI动物都显示脊髓神经元的完整性中断，而组织学显示没有完全损伤的脊柱横向切片。值得注意的是,一些动物在头颅和尾部显示继发性缺血组织的征象。
■这项研究成功地产生了不完全TSCI的猪模型，该模型在72小时内是生理稳定的。我们相信这种TSCI模型将构成潜在的转化模型，以研究人类中TSCI继发的病理生理学。
■是否有可能建立72小时的不完全胸部TSCI镇静猪模型，从而能够连续控制使用，侵入性,在TSCI的整个亚急性期进行非侵入性治疗？
■进行了一项假对照试验以建立模型，和10只动物被分配到假手术或TSCI。所有动物都接受了椎板切除术，TSCI组的动物遭受重量下降损伤。然后将动物镇静72小时。通过基于MRI的离体测量纤维束成像评估损伤量。组织学和免疫组织化学。
■在所有动物中，我们成功地维持了72小时的镇静，而不包括重要的生理参数。基于MRI的纤维束成像显示，所有TSCI动物都显示脊髓神经元的完整性中断，而组织学显示没有完全损伤的脊柱横向切片。值得注意的是,一些动物在头颅和尾部显示继发性缺血组织的征象。
■这项研究成功地产生了不完全TSCI的猪模型，该模型在72小时内是生理稳定的。我们相信这种TSCI模型将构成潜在的转化模型，以研究人类中TSCI继发的病理生理学。
