PDF(Pubmed)
Abstract：
UNASSIGNED: Abrus mollis Hance. (AM) is an important species used in southern Chinese medicine. It is mainly found in Guangdong and Guangxi provinces in China, and it is effective in the treatment of hepatitis. Endophytic bacteria are known to affect the growth and quality of medicinal plants. However, there are limited reports describing endophytic bacteria related to AM.
UNASSIGNED: In the present study, Illumina-based 16S rRNA gene sequencing was used to investigate the endophytic bacterial communities of root nodules of AM at five sampling sites in Guangxi. In addition, 179 strains of endophytic bacteria were isolated and categorized into 13 haplotypes based on recA sequence analysis.
UNASSIGNED: The phylogeny of the 16S rRNA gene sequences revealed a predominance of nonrhizobial endophytes. Microbial diversity analysis showed that Proteobacteria was the dominant phylum in all samples, while Bradyrhizobium was the dominant genus in different samples. An efficient strain, Rhizobium tropici FM-19, was screened and obtained through greenhouse experiments. The AM plants inoculated with this strain showed the best growth performance and high nitrogen fixation and nodulation capacity. Notably, total phenols and total flavonoids, important active components in AM, increased by 30.9 and 42.7%, respectively, after inoculation with Rhizobium tropici FM-19.
UNASSIGNED: This study provides insights into the complex microbial diversity of AM nodules and provides strain information for the efficient cultivation of AM.
UNASSIGNED: In the present study, Illumina-based 16S rRNA gene sequencing was used to investigate the endophytic bacterial communities of root nodules of AM at five sampling sites in Guangxi. In addition, 179 strains of endophytic bacteria were isolated and categorized into 13 haplotypes based on recA sequence analysis.
UNASSIGNED: The phylogeny of the 16S rRNA gene sequences revealed a predominance of nonrhizobial endophytes. Microbial diversity analysis showed that Proteobacteria was the dominant phylum in all samples, while Bradyrhizobium was the dominant genus in different samples. An efficient strain, Rhizobium tropici FM-19, was screened and obtained through greenhouse experiments. The AM plants inoculated with this strain showed the best growth performance and high nitrogen fixation and nodulation capacity. Notably, total phenols and total flavonoids, important active components in AM, increased by 30.9 and 42.7%, respectively, after inoculation with Rhizobium tropici FM-19.
UNASSIGNED: This study provides insights into the complex microbial diversity of AM nodules and provides strain information for the efficient cultivation of AM.
摘要：
■阿布鲁斯·莫利斯·汉斯。(AM)是南方中药中运用的重要品种。它主要在中国的广东和广西两省发现，它对治疗肝炎有效。已知内生细菌会影响药用植物的生长和质量。然而,关于与AM相关的内生细菌的报道有限。
■在本研究中，采用基于Illumina的16SrRNA基因测序技术对广西5个采样点的AM根瘤内生细菌群落进行了研究。此外,根据recA序列分析，分离出179株内生细菌，并将其分为13种单倍型。
■16SrRNA基因序列的系统发育揭示了非根瘤菌内生菌的优势。微生物多样性分析表明,在所有样品中,变形杆菌是优势门,而缓生根瘤菌是不同样品中的优势属。一种高效的菌株，通过温室实验筛选并获得了热带根瘤菌FM-19。接种该菌株的AM植株表现出最佳的生长性能和高的固氮和结瘤能力。值得注意的是,总酚和总黄酮，AM中的重要活性成分，分别增加了30.9%和42.7%，分别,接种热带根瘤菌FM-19后。
■这项研究为AM结节的复杂微生物多样性提供了见解，并为AM的高效培养提供了菌株信息。
■在本研究中，采用基于Illumina的16SrRNA基因测序技术对广西5个采样点的AM根瘤内生细菌群落进行了研究。此外,根据recA序列分析，分离出179株内生细菌，并将其分为13种单倍型。
■16SrRNA基因序列的系统发育揭示了非根瘤菌内生菌的优势。微生物多样性分析表明,在所有样品中,变形杆菌是优势门,而缓生根瘤菌是不同样品中的优势属。一种高效的菌株，通过温室实验筛选并获得了热带根瘤菌FM-19。接种该菌株的AM植株表现出最佳的生长性能和高的固氮和结瘤能力。值得注意的是,总酚和总黄酮，AM中的重要活性成分，分别增加了30.9%和42.7%，分别,接种热带根瘤菌FM-19后。
■这项研究为AM结节的复杂微生物多样性提供了见解，并为AM的高效培养提供了菌株信息。
