Abstract：
UNASSIGNED: To investigate the effects of resveratrol (Res) on human fetal scleral fibroblasts (HFSFs) and its potential mechanism.
UNASSIGNED: HFSFs were randomly divided into the Res-treated group and the control group. Following, HFSFs were treated with or without a concentration of 10 μM Res for 48 h. To detect the expression of related genes, reverse transcription quantitative PCR (RT-qPCR) and western blotting were used. The apoptosis rate of different groups was determined using flow cytometry.
UNASSIGNED: The mRNA expression of matrix metalloproteinase 2 (MMP-2), Collagen, Type I, Alpha 1 (COL1A1), Janus Kinase 2 (JAK2), and Signal Transducer and Activator of Transcription 3 (STAT3)\" was downregulated in the Res-treatment group compared to the control group, according to RT-qPCR. Western blotting revealed that Res therapy reduced the expression of MMP-2, JAK2, P-JAK2, STAT3, P-STAT3, and Bcl-2 associated protein X (Bax) while increasing the expression of COL1A1 and B-cell lymphoma-2 (Bcl-2). Flow cytometry showed that the cell apoptosis rate was significantly lower in HFSFs treated with Res.
UNASSIGNED: In conclusion, these findings suggest that Res increases COL1A1 expression while inhibiting MMP-2 and cell apoptosis in HFSFs, possibly through modulation of the JAK2/STAT3 signaling pathway.
UNASSIGNED: HFSFs were randomly divided into the Res-treated group and the control group. Following, HFSFs were treated with or without a concentration of 10 μM Res for 48 h. To detect the expression of related genes, reverse transcription quantitative PCR (RT-qPCR) and western blotting were used. The apoptosis rate of different groups was determined using flow cytometry.
UNASSIGNED: The mRNA expression of matrix metalloproteinase 2 (MMP-2), Collagen, Type I, Alpha 1 (COL1A1), Janus Kinase 2 (JAK2), and Signal Transducer and Activator of Transcription 3 (STAT3)\" was downregulated in the Res-treatment group compared to the control group, according to RT-qPCR. Western blotting revealed that Res therapy reduced the expression of MMP-2, JAK2, P-JAK2, STAT3, P-STAT3, and Bcl-2 associated protein X (Bax) while increasing the expression of COL1A1 and B-cell lymphoma-2 (Bcl-2). Flow cytometry showed that the cell apoptosis rate was significantly lower in HFSFs treated with Res.
UNASSIGNED: In conclusion, these findings suggest that Res increases COL1A1 expression while inhibiting MMP-2 and cell apoptosis in HFSFs, possibly through modulation of the JAK2/STAT3 signaling pathway.
摘要：
■研究白藜芦醇（Res）对人胎儿巩膜成纤维细胞（HFSFs）的影响及其可能的机制。
■HFSF随机分为Res治疗组和对照组。Follows,HFSF在有或没有浓度为10μMRes的情况下处理48小时。为了检测相关基因的表达，使用逆转录定量PCR(RT-qPCR)和蛋白质印迹。采用流式细胞术测定各组细胞凋亡率。
■基质金属蛋白酶2(MMP-2)mRNA表达,胶原蛋白,I型,Alpha1(COL1A1),Janus激酶2(JAK2),与对照组相比，Res治疗组的信号转导和转录激活因子3（STAT3）”下调，根据RT-qPCR。Westernblot显示，Res治疗降低了MMP-2，JAK2，P-JAK2，STAT3，P-STAT3和Bcl-2相关蛋白X（Bax）的表达，同时增加了COL1A1和B细胞淋巴瘤的表达-2（Bcl-2）。流式细胞仪检测结果显示，Ress处理的HFSFs细胞凋亡率显著降低。
■总而言之，这些发现表明，Res增加COL1A1表达，同时抑制MMP-2和HFSF细胞凋亡，可能通过调节JAK2/STAT3信号通路。
■HFSF随机分为Res治疗组和对照组。Follows,HFSF在有或没有浓度为10μMRes的情况下处理48小时。为了检测相关基因的表达，使用逆转录定量PCR(RT-qPCR)和蛋白质印迹。采用流式细胞术测定各组细胞凋亡率。
■基质金属蛋白酶2(MMP-2)mRNA表达,胶原蛋白,I型,Alpha1(COL1A1),Janus激酶2(JAK2),与对照组相比，Res治疗组的信号转导和转录激活因子3（STAT3）”下调，根据RT-qPCR。Westernblot显示，Res治疗降低了MMP-2，JAK2，P-JAK2，STAT3，P-STAT3和Bcl-2相关蛋白X（Bax）的表达，同时增加了COL1A1和B细胞淋巴瘤的表达-2（Bcl-2）。流式细胞仪检测结果显示，Ress处理的HFSFs细胞凋亡率显著降低。
■总而言之，这些发现表明，Res增加COL1A1表达，同时抑制MMP-2和HFSF细胞凋亡，可能通过调节JAK2/STAT3信号通路。
