Abstract：
We conducted this study to investigate the radioprotective effects of recombinant human thrombopoietin (rhTPO) on beagle dogs irradiated with 3.0 Gy 60Co gamma rays. Fifteen healthy adult beagles were randomly assigned to a control group with alleviating care, and 5 and 10 μg/kg rhTPO treatment group. All animals received total-body irradiation using 60Co γ-ray source at a dose of 3.0 Gy (dose rate was 69.1 cGy/min). The treatment group received intramuscular injection of rhTPO 5 and 10 μg/kg at 2 h postirradiation, and the control group was administrated the same volume of normal saline. The survival rate, clinical signs, peripheral hemogram, serum biochemistry, and histopathological examination of animals in each group were assessed. Single administration of 10 μg/kg rhTPO at 2 h postirradiation promoted the recovery of multilineage hematopoiesis and improved the survival rate of beagles irradiated with 3 Gy 60Co γ rays. The administration of 10 μg/kg rhTPO alleviated fever and bleeding, reduced the requirement for supportive care, and may have mitigated multiple organ damage.
摘要：
我们进行了这项研究，以研究重组人血小板生成素（rhTPO）对3.0Gy60Co伽马射线照射的比格犬的辐射防护作用。15只健康的成年小猎犬被随机分配到对照组，给予缓解护理，以及5和10μg/kgrhTPO治疗组。所有动物均使用60Coγ射线源以3.0Gy的剂量接受全身照射（剂量率为69.1cGy/min）。治疗组放疗后2h肌内注射rhTPO5和10μg/kg，对照组给予等量生理盐水。存活率,临床体征,外周血像，血清生物化学,并对各组动物进行组织病理学检查。辐射后2h单次给药10μg/kgrhTPO促进了多谱系造血的恢复，并提高了3Gy60Coγ射线照射的比格犬的存活率。服用10μg/kgrhTPO可缓解发热和出血，减少了对支持性护理的需求，并可能减轻了多器官损伤。
