Abstract：
Cardiovascular drugs (CVDs) are agents working on the heart and the vascular system to treat many cardiovascular disorders. Such disorders represent the leading cause for morbidity and mortality worldwide. The treatment regimen includes different administered drugs on chronic basis. The cumulative drugs in human body coincides with exposure to electromagnetic radiations from different sources leading to drug-radiation interaction that may lead to drug photosensitization. Such photosensitization may lead to mutagenesis, cancer, and cell death due to molecular damage to DNA. This work involves the application of two bioluminescent genosensors; Terbium chloride and EvaGreen are utilized to investigate potential DNA damage caused by frequently used CVDs following UVA irradiation. A variety of CVDs are investigated. Ten drugs; Amiloride, Atorvastatin, Captopril, Enalapril, Felodipine, Hydrochlorothiazide, Indapamide, Losartan, Triamterene and Valsartan are studied. The study\'s findings showed that such drugs induced DNA damage following UVA irradiation. The induced DNA damage altered the fluorescence of terbium chloride and EvaGreen genosensors, proportionally. The results are confirmed by viscosity measurements reflecting the possible intercalation of CVDs with DNA. Also, the work is applied on calf thymus DNA to mimic the actual biological variability. The demonstrated bioluminescent genosensors provide automatic, simple and low-cost methods for assessing DNA-drug interactions.
摘要：
心血管药物（CVD）是作用于心脏和血管系统的药物，用于治疗许多心血管疾病。这些疾病是全世界发病率和死亡率的主要原因。治疗方案包括基于慢性的不同给药的药物。人体中的累积药物与暴露于来自不同来源的电磁辐射一致，导致药物-辐射相互作用，这可能导致药物的光敏化。这种光敏可能导致诱变，癌症,和DNA分子损伤导致的细胞死亡。这项工作涉及两个生物发光基因传感器的应用；氯化Ter和EvaGreen用于研究UVA辐照后经常使用的CVD引起的潜在DNA损伤。研究了各种CVD。十种药物；阿米洛利，阿托伐他汀,卡托普利,依那普利,非洛地平,氢氯噻嗪,Indapamide,氯沙坦,研究了氨蝶烯和缬沙坦。研究结果表明，这些药物在UVA照射后会引起DNA损伤。诱导的DNA损伤改变了氯化and和EvaGreen基因传感器的荧光，按比例。该结果通过反映CVD与DNA的可能嵌入的粘度测量得到证实。此外，这项工作应用于小牛胸腺DNA，以模拟实际的生物变异性。演示的生物发光基因传感器提供自动，评估DNA-药物相互作用的简单和低成本方法。
