Abstract：
A precise, sensitive, accurate, and validated reverse-phase high-performance liquid chromatography (RP-HPLC) method with a bioanalytical approach was utilized to analyze Cabazitaxel (CBZ) in rat plasma. Comparative research on extraction recoveries was performed between traditional liquid-liquid extraction (LLE) and synthesized graphene oxide (GO) based magnetic solid phase extraction (GO@MSPE). The superparamagnetic hybrid nanosorbent was synthesized using the combination of iron oxide and GO and subsequently applied for extraction and bioanalytical quantification of CBZ from plasma by (HPLC-PDA) analysis. Fourier- transform infrared spectroscopy (FT-IR), particle size, scanning electron microscopy (SEM), and x-ray diffraction (XRD) analysis were employed in the characterization of synthesized GO@MSPE nanosorbent. The investigation was accomplished using a shim pack C18 column (150 mm×4.6 mm, 5 µm) with a binary gradient mobile phase consisting of formic acid: acetonitrile: water (0.1:75:25, v/v/v) at a 0.8 mL/min flow rate, and a λmax of 229 nm. The limits of detection (LOD) and quantitation (LOQ) have been determined to be 50 and 100 ng/mL for both LLE and SPE techniques. The linearity range of the approach encompassed from 100 to 5000 ng/mL and was found to be linear (coefficient of determination > 0.99) for CBZ. The proposed method showed extraction recovery of 76.8-88.4% for the synthesized GO@MSPE and 69.3-77.4% for LLE, suggesting that the proposed bioanalytical approach was robust and qualified for all validation parameters within the acceptable criteria. Furthermore, the developed hybrid GO@MSPE nanosorbent with the help of the proposed RP-HPLC method, showed a significant potential for the extraction of CBZ in bioanalysis.
摘要：
一个精确的，敏感,准确,并采用生物分析方法进行了验证的反相高效液相色谱（RP-HPLC）方法来分析大鼠血浆中的卡巴他赛（CBZ）。对传统液液萃取(LLE)和合成氧化石墨烯(GO)磁性固相萃取(GO@MSPE)的萃取回收率进行了比较研究。使用氧化铁和GO的组合来合成超顺磁性杂化纳米吸附剂,并且随后用于通过(HPLC-PDA)分析从血浆中提取和生物分析定量CBZ。傅里叶变换红外光谱(FT-IR),颗粒大小,扫描电子显微镜（SEM），采用X-射线衍射(XRD)分析对合成的GO@MSPE纳米吸附剂进行了表征。使用垫片包C18柱(150mm×4.6mm，5µm），具有由甲酸：乙腈：水（0.1：75：25，v/v/v）组成的二元梯度流动相，流速为0.8mL/min，和229nm的λmax。LLE和SPE技术的检测限（LOD）和定量限（LOQ）已确定为50和100ng/mL。该方法的线性范围涵盖100至5000ng/mL,并且发现对于CBZ是线性的(测定系数>0.99)。所提出的方法显示，合成的GO@MSPE的提取回收率为76.8-88.4%，LLE的提取回收率为69.3-77.4%，这表明所提出的生物分析方法是稳健的，并且在可接受的标准内符合所有验证参数。此外，在提出的RP-HPLC方法的帮助下，开发的杂化GO@MSPE纳米吸附剂，显示了在生物分析中提取CBZ的显着潜力。
