Abstract：
Dermal papilla cell (DPC) belongs to a specialized mesenchymal stem cell for hair follicle regeneration. Maintaining the ability of DPCs to stimulate hair in vitro culture is important for hair follicle morphogenesis and regeneration. As the third generation of platelet concentrate, injectable platelet-rich fibrin (i-PRF) is a novel biomaterial containing many growth factors and showing promising effects on tissue reconstruction. We aimed to explore the influences of i-PRF on the proliferative, migratory, as well as trichogenic ability of DPCs and compared the effects of i-PRF and platelet-rich plasma (PRP), the first generation of platelet concentrate. Both PRP and i-PRF facilitated DPCs proliferation, and migration, along with trichogenic inductivity as well as stimulated the TGF-β/Smad pathway, while the impacts of i-PRF were more significant than PRP. A small molecule inhibitor of TGF-beta receptor I, Galunisertib, was also applied to treat DPCs, and it rescued the impacts of i-PRF on the proliferative, migratory, trichogenic inductivity, and proteins-associated with TGF-β/Smad pathway in DPCs. These findings revealed that i-PRF had better effects than PRP in enhancing the proliferative, migratory, and hair-inducing abilities of DPCs by the TGF-β/Smad pathway, which indicated the beneficial role of i-PRF in hair follicle regeneration.
摘要：
毛乳头细胞(DPC)是一种特殊的毛囊再生间充质干细胞。保持DPC在体外培养中刺激毛发的能力对于毛囊形态发生和再生是重要的。作为第三代浓缩血小板，可注射富血小板纤维蛋白（i-PRF）是一种新型生物材料，含有许多生长因子，对组织重建显示出有希望的效果。我们旨在探讨i-PRF对增殖的影响，迁徙,以及DPCs的致毛能力，并比较了i-PRF和富血小板血浆（PRP）的作用，第一代浓缩血小板。PRP和i-PRF都促进了DPC的增殖，和移民,随着毛致诱导以及刺激TGF-β/Smad途径，而i-PRF的影响比PRP更显著。TGF-β受体I的小分子抑制剂,Galunisertib,还用于治疗DPC，它拯救了i-PRF对增殖的影响，迁徙,致毛诱导，以及与DPCs中TGF-β/Smad途径相关的蛋白质。这些发现表明，i-PRF在增强增殖方面比PRP具有更好的效果，迁徙,和通过TGF-β/Smad途径诱导DPCs的毛发能力，这表明i-PRF在毛囊再生中的有益作用。
