PDF(Pubmed)
Abstract：
BACKGROUND: Ovarian cancer (OC) is a malignancy among female globally. Circular RNAs (circRNAs) are a family of circular endogenous RNAs generated from selective splicing, which take part in many traits. Former investigation suggested that circ-TFRC was abnormally expressed in breast cancer (BC). Further, the role of circ-TFRC to the progress of OC remains unclear. So, the aim of this study was to reveal the regulatory mechanism of circ-TFRC.
METHODS: Our team made the luciferase reporter assay to validate circ-TFRC downstream target. Transwell migration assay, 5-ethynyl-20-deoxyuridine, and cell counting kit-8 were applied to investigate both proliferation and migration. In vivo tumorigenesis and metastasis assays were performed to investigate the circ-TFRC role in OC.
RESULTS: The outputs elucidated that circ-TFRC expression incremented in OC cells and tissues. circ-TFRC downregulation inhibited OC cell proliferation as well as migration in in vivo and in vitro experiments. The luciferase results validated that miR-615-3p and IGF2 were circ-TFRC downstream targets. IGF2 overexpression or miR-615-3p inhibition reversed OC cell migration after circ-TFRC silencing. Also, IGF2 overexpression reversed OC cell migration and proliferation post miR-615-3p upregulation.
CONCLUSIONS: Results demonstrate that circ-TFRC downregulation inhibits OC progression and metastasis via IGF2 expression regulation and miR-615-3psponging.
METHODS: Our team made the luciferase reporter assay to validate circ-TFRC downstream target. Transwell migration assay, 5-ethynyl-20-deoxyuridine, and cell counting kit-8 were applied to investigate both proliferation and migration. In vivo tumorigenesis and metastasis assays were performed to investigate the circ-TFRC role in OC.
RESULTS: The outputs elucidated that circ-TFRC expression incremented in OC cells and tissues. circ-TFRC downregulation inhibited OC cell proliferation as well as migration in in vivo and in vitro experiments. The luciferase results validated that miR-615-3p and IGF2 were circ-TFRC downstream targets. IGF2 overexpression or miR-615-3p inhibition reversed OC cell migration after circ-TFRC silencing. Also, IGF2 overexpression reversed OC cell migration and proliferation post miR-615-3p upregulation.
CONCLUSIONS: Results demonstrate that circ-TFRC downregulation inhibits OC progression and metastasis via IGF2 expression regulation and miR-615-3psponging.
摘要：
背景：卵巢癌（OC）是全球女性的恶性肿瘤。环状RNA（circularRNAs）是由选择性剪接产生的环状内源性RNAs家族，参与许多特征。以前的研究表明circ-TFRC在乳腺癌（BC）中异常表达。Further,Circ-TFRC对OC进展的作用尚不清楚。所以,本研究旨在揭示circ-TFRC的调控机制。
方法：我们的团队进行了荧光素酶报告基因测定以验证circ-TFRC下游靶标。Transwell迁移试验，5-乙炔基-20-脱氧尿苷,和细胞计数试剂盒-8用于研究增殖和迁移。进行体内肿瘤发生和转移测定以研究circ-TFRC在OC中的作用。
结果:输出阐明circ-TFRC表达在OC细胞和组织中增加。在体内和体外实验中，circ-TFRC下调抑制OC细胞增殖以及迁移。荧光素酶结果验证miR-615-3p和IGF2是circ-TFRC下游靶标。IGF2过表达或miR-615-3p抑制在circ-TFRC沉默后逆转OC细胞迁移。此外，IGF2过表达在miR-615-3p上调后逆转OC细胞迁移和增殖。
结论：结果表明，circ-TFRC下调通过IGF2表达调控和miR-615-3psponging抑制OC进展和转移。
方法：我们的团队进行了荧光素酶报告基因测定以验证circ-TFRC下游靶标。Transwell迁移试验，5-乙炔基-20-脱氧尿苷,和细胞计数试剂盒-8用于研究增殖和迁移。进行体内肿瘤发生和转移测定以研究circ-TFRC在OC中的作用。
结果:输出阐明circ-TFRC表达在OC细胞和组织中增加。在体内和体外实验中，circ-TFRC下调抑制OC细胞增殖以及迁移。荧光素酶结果验证miR-615-3p和IGF2是circ-TFRC下游靶标。IGF2过表达或miR-615-3p抑制在circ-TFRC沉默后逆转OC细胞迁移。此外，IGF2过表达在miR-615-3p上调后逆转OC细胞迁移和增殖。
结论：结果表明，circ-TFRC下调通过IGF2表达调控和miR-615-3psponging抑制OC进展和转移。
