Abstract：
OBJECTIVE: Renal cell carcinoma (RCC) presents a formidable clinical challenge due to its aggressive behavior and limited therapeutic options. Matrix metalloproteinase-8 (MMP-8) has recently emerged as a potential biomarker and therapeutic target for various cancers. However, the genetic involvement of MMP-8 in RCC has remained largely obscure. This study aimed to elucidate the role of MMP-8 genotypes in RCC susceptibility.
METHODS: The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was employed to scrutinize the genotypes of MMP-8 C-799T (rs11225395), Val436Ala (rs34009635), and Lys460Thr (rs35866072) among 118 RCC patients and 590 controls. Furthermore, potential associations between MMP-8 genotypes and age, sex, smoking, alcohol consumption, hypertension, diabetes, and family history status in relation to RCC risk were assessed.
RESULTS: No significant disparities in the distribution of MMP-8 rs11225395, rs34009635, and rs35866072 genotypes were observed between the RCC case and control cohorts (p>0.05). Individuals with CT and TT genotypes at MMP-8 rs11225395 exhibited 0.86- and 0.80-fold RCC risks, respectively (OR=0.57-1.31 and 0.42-1.55, p=0.5585 and 0.6228, respectively). Intriguingly, hypertensive individuals carrying the MMP-8 rs11225395 CT or TT genotype demonstrated an elevated risk for RCC compared to those with wild-type CC genotype (p=0.0440). No interactions of MMP-8 genotypes with age, sex, smoking, alcohol consumption, or diabetes status were evident (all p>0.05). No significant association was discerned for MMP-8 rs34009635 or rs35866072 genotypes.
CONCLUSIONS: MMP-8 genotypes appear to have a modest influence on individual susceptibility to RCC. Hypertensive patients with the CT or TT MMP-8 rs11225395 genotype may have an elevated risk of RCC.
METHODS: The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was employed to scrutinize the genotypes of MMP-8 C-799T (rs11225395), Val436Ala (rs34009635), and Lys460Thr (rs35866072) among 118 RCC patients and 590 controls. Furthermore, potential associations between MMP-8 genotypes and age, sex, smoking, alcohol consumption, hypertension, diabetes, and family history status in relation to RCC risk were assessed.
RESULTS: No significant disparities in the distribution of MMP-8 rs11225395, rs34009635, and rs35866072 genotypes were observed between the RCC case and control cohorts (p>0.05). Individuals with CT and TT genotypes at MMP-8 rs11225395 exhibited 0.86- and 0.80-fold RCC risks, respectively (OR=0.57-1.31 and 0.42-1.55, p=0.5585 and 0.6228, respectively). Intriguingly, hypertensive individuals carrying the MMP-8 rs11225395 CT or TT genotype demonstrated an elevated risk for RCC compared to those with wild-type CC genotype (p=0.0440). No interactions of MMP-8 genotypes with age, sex, smoking, alcohol consumption, or diabetes status were evident (all p>0.05). No significant association was discerned for MMP-8 rs34009635 or rs35866072 genotypes.
CONCLUSIONS: MMP-8 genotypes appear to have a modest influence on individual susceptibility to RCC. Hypertensive patients with the CT or TT MMP-8 rs11225395 genotype may have an elevated risk of RCC.
摘要：
目的：肾细胞癌（RCC）由于其攻击行为和有限的治疗选择而提出了巨大的临床挑战。基质金属蛋白酶-8（MMP-8）最近已成为各种癌症的潜在生物标志物和治疗靶标。然而,MMP-8在RCC中的遗传参与在很大程度上仍然不清楚。本研究旨在阐明MMP-8基因型在RCC易感性中的作用。
方法：采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术来审查MMP-8C-799T（rs11225395）的基因型，Val436Ala(rs34009635),118例RCC患者和590例对照者中的Lys460Thr(rs35866072)。此外，MMP-8基因型与年龄之间的潜在关联，性别,吸烟,酒精消费,高血压,糖尿病,并评估了与RCC风险相关的家族史。
结果：在RCC病例组和对照组之间，MMP-8rs11225395、rs34009635和rs35866072基因型的分布没有显著差异（p>0.05）。在MMP-8rs11225395处具有CT和TT基因型的个体表现出0.86倍和0.80倍的RCC风险，（OR分别为0.57-1.31和0.42-1.55，p分别为0.5585和0.6228）。有趣的是，与野生型CC基因型患者相比,携带MMP-8rs11225395CT或TT基因型的高血压患者患RCC的风险较高(p=0.0440).MMP-8基因型与年龄无关，性别,吸烟,酒精消费,或糖尿病状态明显（均p>0.05）。MMP-8rs34009635或rs35866072基因型没有发现显著的关联。
结论：MMP-8基因型似乎对个体对RCC的易感性有适度的影响。CT或TTMMP-8rs11225395基因型的高血压患者可能具有升高的RCC风险。
方法：采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术来审查MMP-8C-799T（rs11225395）的基因型，Val436Ala(rs34009635),118例RCC患者和590例对照者中的Lys460Thr(rs35866072)。此外，MMP-8基因型与年龄之间的潜在关联，性别,吸烟,酒精消费,高血压,糖尿病,并评估了与RCC风险相关的家族史。
结果：在RCC病例组和对照组之间，MMP-8rs11225395、rs34009635和rs35866072基因型的分布没有显著差异（p>0.05）。在MMP-8rs11225395处具有CT和TT基因型的个体表现出0.86倍和0.80倍的RCC风险，（OR分别为0.57-1.31和0.42-1.55，p分别为0.5585和0.6228）。有趣的是，与野生型CC基因型患者相比,携带MMP-8rs11225395CT或TT基因型的高血压患者患RCC的风险较高(p=0.0440).MMP-8基因型与年龄无关，性别,吸烟,酒精消费,或糖尿病状态明显（均p>0.05）。MMP-8rs34009635或rs35866072基因型没有发现显著的关联。
结论：MMP-8基因型似乎对个体对RCC的易感性有适度的影响。CT或TTMMP-8rs11225395基因型的高血压患者可能具有升高的RCC风险。
